Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 99551..100076 Replicon plasmid p1606A
Accession NZ_CP083702
Organism Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag K9O95_RS25170 Protein ID WP_001159871.1
Coordinates 99551..99856 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag K9O95_RS25175 Protein ID WP_000813630.1
Coordinates 99858..100076 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9O95_RS25155 (95513) 95513..96679 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
K9O95_RS25160 (97267) 97267..98022 - 756 WP_000852146.1 replication initiation protein RepE -
K9O95_RS25165 (98744) 98744..99550 - 807 WP_000016970.1 site-specific integrase -
K9O95_RS25170 (99551) 99551..99856 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
K9O95_RS25175 (99858) 99858..100076 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K9O95_RS25180 (100636) 100636..100866 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
K9O95_RS25185 (100863) 100863..101279 + 417 WP_224451506.1 type II toxin-antitoxin system VapC family toxin -
K9O95_RS25190 (101354) 101354..102919 + 1566 WP_001128474.1 AAA family ATPase -
K9O95_RS25195 (102904) 102904..103926 + 1023 WP_000361402.1 helicase UvrD -
K9O95_RS25200 (104180) 104180..104866 - 687 Protein_123 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / tet(A) - 1..120731 120731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T217124 WP_001159871.1 NZ_CP083702:c99856-99551 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T217124 NZ_CP113837:4429922-4430176 [Kitasatospora sp. YST-16]
GTGAGGCTCGTATTCGAAGAGCAGGGCTGGGAGGACTACACGTCCTGGCTCAAGAGCGATCGCAAGATGCTCGCGAGGAT
CAACAAGCTCATCGAGGATGTCCTGCGCGATCCGTTCACCGGGATCGGCAAGCCCGAGCCGCTGAAGTACCACCTGCCCG
GGGCCTGGTCGCGGCGGATCGATGACGAGCATCGCCTCGTCTATCTGGTCACCGACCGGGAAATCATCATCCTGGCCGCC
CGCTATCACTATTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT217124 WP_000813630.1 NZ_CP083702:c100076-99858 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT217124 NZ_CP113837:4429644-4429925 [Kitasatospora sp. YST-16]
ATGTCCATCACCGCGAGCGAGGCACGCAGGGATCTCTTCCCGTTGATCAAGAAGGTCAACGAGGATCATGACGCCATCGA
GATCGTGTCCAAGCACGGAAATGCGGTGCTCGTCTCCGCCGAGGATTACGCCTCGTTGCGCGAGGGCTCCTATCTCTTGC
GTTCGCCGGCGAACGCGCGTCGCCTGCTGAGGGCGTACGACAATGCGCTGAACGGCATAGAGCTCTCCGAGCGCACGCTC
ATCGACCCCGACTCGGCTGATGCCGGGCGGGGTGTCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References