Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4762537..4763120 Replicon chromosome
Accession NZ_CP083701
Organism Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag K9O95_RS23280 Protein ID WP_061349013.1
Coordinates 4762785..4763120 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag K9O95_RS23275 Protein ID WP_000581937.1
Coordinates 4762537..4762785 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9O95_RS23265 (4758876) 4758876..4760177 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
K9O95_RS23270 (4760225) 4760225..4762459 + 2235 WP_000226815.1 GTP pyrophosphokinase -
K9O95_RS23275 (4762537) 4762537..4762785 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
K9O95_RS23280 (4762785) 4762785..4763120 + 336 WP_061349013.1 endoribonuclease MazF Toxin
K9O95_RS23285 (4763191) 4763191..4763982 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
K9O95_RS23290 (4764210) 4764210..4765847 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
K9O95_RS23295 (4765935) 4765935..4767233 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12112.07 Da        Isoelectric Point: 8.2618

>T217117 WP_061349013.1 NZ_CP083701:4762785-4763120 [Escherichia coli]
MVSRYVPDMGDLIWIDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T217117 NZ_CP113832:2689483-2689626 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT217117 WP_000581937.1 NZ_CP083701:4762537-4762785 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT217117 NZ_CP113832:c2689817-2689558 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATA
ATAGCATTGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACTTTTTGGATTATTT
AAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCTTCTTTAACAACCTTTAGGATTCCGAAGATTAAA
GTCGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References