Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3187713..3188210 Replicon chromosome
Accession NZ_CP083701
Organism Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K9O95_RS15750 Protein ID WP_001173343.1
Coordinates 3187713..3187886 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K9O95_RS15755 Protein ID WP_000398619.1
Coordinates 3187914..3188210 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9O95_RS15740 (3184378) 3184378..3185835 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
K9O95_RS15745 (3186072) 3186072..3187589 + 1518 WP_001295074.1 lysine--tRNA ligase -
K9O95_RS15750 (3187713) 3187713..3187886 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K9O95_RS15755 (3187914) 3187914..3188210 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K9O95_RS15760 (3188437) 3188437..3188709 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
K9O95_RS15765 (3188721) 3188721..3188951 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
K9O95_RS15770 (3189132) 3189132..3190763 + 1632 WP_001216477.1 sensor histidine kinase -
K9O95_RS15775 (3190760) 3190760..3191479 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T217111 WP_001173343.1 NZ_CP083701:c3187886-3187713 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T217111 NZ_CP113832:c2548240-2548139 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT217111 WP_000398619.1 NZ_CP083701:c3188210-3187914 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT217111 NZ_CP113832:2548015-2548202 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGTGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAACGCAACAAGGGTTGCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References