Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2568177..2569012 Replicon chromosome
Accession NZ_CP083701
Organism Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)


Gene name yeeV Uniprot ID S1EZ92
Locus tag K9O95_RS12795 Protein ID WP_000854726.1
Coordinates 2568177..2568554 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1L6W4Z7
Locus tag K9O95_RS12800 Protein ID WP_070749977.1
Coordinates 2568644..2569012 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9O95_RS12765 (2563967) 2563967..2564815 - 849 WP_001280510.1 DUF4942 domain-containing protein -
K9O95_RS12770 (2564900) 2564900..2565097 - 198 WP_000839267.1 DUF957 domain-containing protein -
K9O95_RS12775 (2565109) 2565109..2565519 - 411 Protein_2512 DUF5983 family protein -
K9O95_RS12780 (2565713) 2565713..2567252 + 1540 Protein_2513 IS21-like element ISEc12 family transposase -
K9O95_RS12785 (2567267) 2567267..2568012 + 746 Protein_2514 IS21-like element ISEc12 family helper ATPase IstB -
K9O95_RS12790 (2568097) 2568097..2568180 - 84 Protein_2515 DUF5983 family protein -
K9O95_RS12795 (2568177) 2568177..2568554 - 378 WP_000854726.1 TA system toxin CbtA family protein Toxin
K9O95_RS12800 (2568644) 2568644..2569012 - 369 WP_070749977.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K9O95_RS12805 (2569062) 2569062..2569706 - 645 Protein_2518 antitoxin of toxin-antitoxin stability system -
K9O95_RS12810 (2569721) 2569721..2569942 - 222 WP_000692353.1 DUF987 domain-containing protein -
K9O95_RS12815 (2570011) 2570011..2570487 - 477 WP_001186742.1 RadC family protein -
K9O95_RS12820 (2570501) 2570501..2570980 - 480 WP_000706976.1 antirestriction protein -
K9O95_RS12825 (2571074) 2571074..2571319 - 246 WP_000680584.1 hypothetical protein -
K9O95_RS12830 (2571319) 2571319..2572137 - 819 WP_070749978.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage ant(3'')-Ia / blaOXA-1 / qepA1 / catA1 - 2495926..2626817 130891


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14164.10 Da        Isoelectric Point: 7.8045

>T217107 WP_000854726.1 NZ_CP083701:c2568554-2568177 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Download         Length: 378 bp

>T217107 NZ_CP113832:c339002-338907 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCTATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13860.63 Da        Isoelectric Point: 6.3177

>AT217107 WP_070749977.1 NZ_CP083701:c2569012-2568644 [Escherichia coli]
VSDTFHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQVFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT217107 NZ_CP113832:338807-338872 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EZ92

Antitoxin

Source ID Structure
AlphaFold DB A0A1L6W4Z7

References