Detailed information of TA system TA217094

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 556155..556990
• Replicon: chromosome
• Accession: NZ_CP083701
• Organism: Escherichia coli strain SUISSEKPC3NDM5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K9O95_RS02665
• Protein ID: WP_064221650.1
• Coordinates: 556155..556532 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: K9O95_RS02670
• Protein ID: WP_001295723.1
• Coordinates: 556622..556990 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9O95_RS02630 (551675)	551675..552148	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
K9O95_RS02635 (552346)	552346..553404	+	1059	WP_001200895.1	FUSC family protein	-
K9O95_RS02640 (553576)	553576..553905	+	330	WP_000450409.1	DUF496 family protein	-
K9O95_RS02645 (554006)	554006..554371	-	366	WP_001280454.1	EutP/PduV family microcompartment system protein	-
K9O95_RS02650 (554642)	554642..555031	-	390	WP_077875581.1	transposase	-
K9O95_RS02655 (555829)	555829..555909	-	81	Protein_521	hypothetical protein	-
K9O95_RS02660 (556009)	556009..556158	-	150	Protein_522	DUF5983 family protein	-
K9O95_RS02665 (556155)	556155..556532	-	378	WP_064221650.1	TA system toxin CbtA family protein	Toxin
K9O95_RS02670 (556622)	556622..556990	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K9O95_RS02675 (557040)	557040..557177	-	138	WP_168787260.1	hypothetical protein	-
K9O95_RS02680 (557358)	557358..558897	+	1540	Protein_526	IS21-like element ISEc12 family transposase	-
K9O95_RS02685 (558912)	558912..559655	+	744	WP_224451502.1	IS21-like element ISEc12 family helper ATPase IstB	-
K9O95_RS02690 (559713)	559713..559955	-	243	WP_049067502.1	DUF987 domain-containing protein	-
K9O95_RS02695 (560018)	560018..560494	-	477	WP_001186775.1	RadC family protein	-
K9O95_RS02700 (560510)	560510..560995	-	486	WP_000849588.1	antirestriction protein	-
K9O95_RS02705 (561050)	561050..561868	-	819	WP_072649824.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14150.14 Da
• Isoelectric Point: 7.3249

>T217094 WP_064221650.1 NZ_CP083701:c556532-556155 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTQLARLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T217094 NZ_CP113827:5163825-5164115 [Pandoraea sp. XJJ-1]
TTGACGACCTATGACCTGCAGTTCCTACCCTCTGCCCTCAAGGAGTGGCAGGCGCTCGATTCCACGATCCAGCGCCAGTT
CAAAGCGAAGTTGCAGCAACGGCTGCAAAGACCCCAAGTTCCCGGCAGTCGGCTAAGGTCGATGCCGGACTGCTACAAGA
TCAAACTCGCATCGGTGGGATACCGGCTCGTCTACCGAGTCGACGACAAAGCCGTCACCGTGTTGGTGGTCGCCGTCGGC
AAACGAGAGCACCTTGCGACTTATCGCACGGCCCGAAAACGCGTTCAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT217094 WP_001295723.1 NZ_CP083701:c556990-556622 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT217094 NZ_CP113827:5163596-5163838 [Pandoraea sp. XJJ-1]
ATGAAAAACATCCTTGCCCGCGCATCGATCGGAATCAGCGAATTGAAATCGAACCCCAGTGCCGCCATCCATCGAGCCGA
TGGCCCCGTCGCCATCCTGAACCGGAACACGCCGGTCGCCTATCTCGTCCCCGCAGCGGATTGGGAAGCGATCTGCGAGC
GGCTCGATGACGCTGAACTCGCCGACCTTATCCGTTCACGCGCCGGCGAAACGCCGGTGAGTACCCAGCTTGACGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: S1NM52