Detailed information of TA system TA217083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2652539..2653140
• Replicon: chromosome
• Accession: NZ_CP083690
• Organism: Serratia sp. HRI

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GF111_RS12775
• Protein ID: WP_055317375.1
• Coordinates: 2652760..2653140 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0G3SPC0
• Locus tag: GF111_RS12770
• Protein ID: WP_038875999.1
• Coordinates: 2652539..2652760 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GF111_RS12750 (GF111_12750)	2647688..2648665	+	978	WP_055317377.1	glyoxylate/hydroxypyruvate reductase GhrB	-
GF111_RS12755 (GF111_12755)	2648667..2648819	-	153	WP_168166338.1	hypothetical protein	-
GF111_RS12760 (GF111_12760)	2649015..2651078	+	2064	WP_038875997.1	alpha-amylase	-
GF111_RS12765 (GF111_12765)	2651214..2652464	+	1251	WP_004933935.1	valine--pyruvate transaminase	-
GF111_RS12770 (GF111_12770)	2652539..2652760	+	222	WP_038875999.1	ribbon-helix-helix domain-containing protein	Antitoxin
GF111_RS12775 (GF111_12775)	2652760..2653140	+	381	WP_055317375.1	type II toxin-antitoxin system VapC family toxin	Toxin
GF111_RS12780 (GF111_12780)	2653151..2654809	-	1659	WP_047729572.1	putative transporter	-
GF111_RS12785 (GF111_12785)	2654977..2655405	-	429	WP_147190061.1	small heat shock chaperone IbpB	-
GF111_RS12790 (GF111_12790)	2655506..2655919	-	414	WP_004933919.1	small heat shock chaperone IbpA	-
GF111_RS12795 (GF111_12795)	2656229..2656576	+	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
GF111_RS12800 (GF111_12800)	2656607..2657887	-	1281	WP_147190059.1	DUF3748 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14215.56 Da
• Isoelectric Point: 7.3170

>T217083 WP_055317375.1 NZ_CP083690:2652760-2653140 [Serratia sp. HRI]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPTLELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T217083 NZ_CP113799:c352327-352070 [Rhodococcus pyridinivorans]
GTGAAAACGATGAGTTACACCGAATCGCGGGCACGCTACGCCGAGGTCCTCGACGCAGTGACCAATGACCGCGAGGAAGT
CATCATCACCCGTGCCGGCCACGAACCCGTGGTCATGGTCTCCCTCGATGATTACGAGTCCCTCCGGGAGACCGCGCACC
TGATGCGCTCCCCCGCCAACGCTCGTCGCCTCTTGGACGCAATGGAGAGGCTCGAAGCCGGTCGCGGCCAGTCGCACAGC
CTGCTCGACGTCGACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8563.50 Da
• Isoelectric Point: 4.4791

>AT217083 WP_038875999.1 NZ_CP083690:2652539-2652760 [Serratia sp. HRI]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLAENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT217083 NZ_CP113799:c352068-351811 [Rhodococcus pyridinivorans]
ATGCTGTTGGTGTGGGACGAGAACGCCTGGCAGGACTACCTTTGGTGGCAACAACAGGACCGAAAGATCCTCAAGAGGAT
CAATACCCTACTGGTCGATATCCAGCGCAATGGCAATGAGGGAATCGGCAAACCCGAACCTCTCAAGTACGACTTCGCCG
GGTATTGGTCCAGACGAATCACCGACGAGCACCGGCTTGTTTACAAGGTGACCGACACGGAGGTCCGCATCGCCGCCTGT
CGGTACCACTACGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0G3SPC0