Detailed information of TA system TA217077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 995295..995842
• Replicon: chromosome
• Accession: NZ_CP083690
• Organism: Serratia sp. HRI

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GF111_RS04810
• Protein ID: WP_047728145.1
• Coordinates: 995295..995603 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0G3SUU9
• Locus tag: GF111_RS04815
• Protein ID: WP_033633717.1
• Coordinates: 995606..995842 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GF111_RS04790 (GF111_04790)	991063..992220	-	1158	WP_047728141.1	YbfB/YjiJ family MFS transporter	-
GF111_RS04795 (GF111_04795)	992326..993246	+	921	WP_038880964.1	LysR family transcriptional regulator	-
GF111_RS04800 (GF111_04800)	993277..994401	+	1125	WP_038880962.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
GF111_RS04805 (GF111_04805)	994416..995258	+	843	WP_047728144.1	S-formylglutathione hydrolase	-
GF111_RS04810 (GF111_04810)	995295..995603	-	309	WP_047728145.1	CcdB family protein	Toxin
GF111_RS04815 (GF111_04815)	995606..995842	-	237	WP_033633717.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GF111_RS04820 (GF111_04820)	995930..996838	-	909	WP_171887866.1	glutathione ABC transporter permease GsiD	-
GF111_RS04825 (GF111_04825)	996848..997768	-	921	WP_038880960.1	glutathione ABC transporter permease GsiC	-
GF111_RS04830 (GF111_04830)	997821..999356	-	1536	WP_055312408.1	glutathione ABC transporter substrate-binding protein GsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11472.26 Da
• Isoelectric Point: 5.0196

>T217077 WP_047728145.1 NZ_CP083690:c995603-995295 [Serratia sp. HRI]
MQFTVYDNTYPASAYPYLLDVQSDLIDVLSTRLVIPLYALDNVRVKISARLCPEIEVNGEKFLVMTHEMAAVRISQIGKA
VGNVNEHRNQIKAAIDFLIDGF

Nucleotide

• Length: 309 bp

>T217077 NZ_CP113792:54817-55122 [Klebsiella variicola subsp. tropica]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACTTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8994.07 Da
• Isoelectric Point: 5.0807

>AT217077 WP_033633717.1 NZ_CP083690:c995842-995606 [Serratia sp. HRI]
MANNNVTKKTVNVTIDRDLFQRAKSLGVNVSSVLTDALSVRVRDIEIQQWREQNRAALEELNRITEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT217077 NZ_CP113792:54597-54815 [Klebsiella variicola subsp. tropica]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGATTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0G3SUU9