Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2104044..2104573 Replicon chromosome
Accession NZ_CP083671
Organism Staphylococcus aureus strain HL25870

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag K3A99_RS10410 Protein ID WP_000621175.1
Coordinates 2104044..2104406 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag K3A99_RS10415 Protein ID WP_000948331.1
Coordinates 2104403..2104573 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3A99_RS10390 (2101022) 2101022..2101792 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
K3A99_RS10395 (2101767) 2101767..2102246 - 480 WP_001190825.1 anti-sigma B factor RsbW -
K3A99_RS10400 (2102248) 2102248..2102574 - 327 WP_001052491.1 anti-sigma factor antagonist -
K3A99_RS10405 (2102693) 2102693..2103694 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
K3A99_RS10410 (2104044) 2104044..2104406 - 363 WP_000621175.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K3A99_RS10415 (2104403) 2104403..2104573 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
K3A99_RS10420 (2104658) 2104658..2105806 - 1149 WP_001281139.1 alanine racemase -
K3A99_RS10425 (2105872) 2105872..2106231 - 360 WP_000581197.1 holo-ACP synthase -
K3A99_RS10430 (2106235) 2106235..2106726 - 492 WP_001286980.1 PH domain-containing protein -
K3A99_RS10435 (2106719) 2106719..2108296 - 1578 WP_001294650.1 PH domain-containing protein -
K3A99_RS10440 (2108289) 2108289..2108768 - 480 WP_001287081.1 hypothetical protein -
K3A99_RS10445 (2108977) 2108977..2109537 - 561 WP_001092419.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13441.69 Da        Isoelectric Point: 10.1654

>T217062 WP_000621175.1 NZ_CP083671:c2104406-2104044 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Download         Length: 363 bp

>T217062 NZ_CP113784:c4066321-4066218 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT217062 WP_000948331.1 NZ_CP083671:c2104573-2104403 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT217062 NZ_CP113784:4066213-4066356 [Citrobacter freundii]
CAAATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References