Detailed information of TA system TA021705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 226551..228096
• Replicon: plasmid pRL10
• Accession: NC_008381
• Organism: Rhizobium leguminosarum bv. viciae 3841

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A8G2ITU5
• Locus tag: RL_RS28495
• Protein ID: WP_028743217.1
• Coordinates: 227161..228096 (+)
• Length: 312 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: Q1M7T2
• Locus tag: RL_RS28490
• Protein ID: WP_011654254.1
• Coordinates: 226551..227171 (+)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RL_RS28480	222185..222496	-	312	WP_011654252.1	hypothetical protein	-
RL_RS28485	222784..226068	+	3285	WP_011654253.1	Ti-type conjugative transfer relaxase TraA	-
RL_RS28490	226551..227171	+	621	WP_011654254.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
RL_RS28495	227161..228096	+	936	WP_028743217.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
RL_RS28500	228115..228416	-	302	Protein_224	type II toxin-antitoxin system RelE/ParE family toxin	-
RL_RS28505	228416..228697	-	282	WP_011654256.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
RL_RS28510	228918..229910	+	993	WP_011654257.1	LLM class flavin-dependent oxidoreductase	-
RL_RS28515	229936..231285	+	1350	WP_011654258.1	LLM class flavin-dependent oxidoreductase	-
RL_RS28520	231303..232502	+	1200	WP_011654259.1	amidohydrolase	-
RL_RS28525	232499..233056	+	558	WP_011654260.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..488135	488135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-272)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (13-56)

Sequences

Toxin

Protein

• Length: 312 a.a.
• Molecular weight: 34989.22 Da
• Isoelectric Point: 6.5223

>T21705 WP_028743217.1 NC_008381:227161-228096 [Rhizobium leguminosarum bv. viciae 3841]
MARELKKNVGASVRARLLDRSRAERTDFQILLTRYALERLLYRLSLSEHRDRFILKGAMLFVTWVADPFRPTRDLDLLGY
GENSPKAIAETFHAICTQPVDADGVVFDVDALEAAPIREEVEYGGVRVRTTATIGGARIPIQVDIGFGDAVTPGPVDIEY
PSLLDAPVPRLRAYPVATVVAEKFEALVTLGMANSRLKDFYDLWLIAETFELERSTLAEAVRQTFARRGTGIPMERPTGL
SEAYAEAWDRQWRAFLGRERMAAAPPALGTVVADLGRFLLPLTQPVEGEWRWKPYEGWIQLQVKQLGYPKR

Nucleotide

• Length: 936 bp

>T21705 NC_008381:227161-228096 [Rhizobium leguminosarum bv. viciae 3841]
ATGGCGCGTGAGCTCAAGAAAAACGTAGGGGCATCGGTGCGCGCCCGGCTGCTGGATCGGTCTCGCGCAGAGCGTACGGA
CTTTCAGATCCTACTGACGCGCTACGCGCTCGAAAGACTCCTGTACCGGCTGAGCCTATCGGAGCATCGCGACCGCTTTA
TCCTGAAGGGCGCGATGCTGTTCGTAACCTGGGTCGCCGACCCGTTTCGGCCGACGCGGGACCTCGATCTGCTGGGCTAC
GGCGAAAACAGCCCGAAGGCCATCGCCGAAACTTTCCATGCCATTTGCACGCAGCCGGTCGACGCTGATGGCGTGGTCTT
CGACGTGGATGCACTCGAAGCCGCGCCGATCCGTGAGGAAGTCGAATATGGCGGCGTTCGTGTTCGTACCACTGCCACCA
TCGGAGGCGCGCGCATTCCCATTCAGGTGGACATAGGTTTCGGTGACGCCGTCACGCCTGGACCGGTCGACATAGAATAT
CCGTCGCTACTCGATGCGCCGGTGCCGCGCCTGCGAGCCTATCCGGTCGCGACCGTGGTCGCCGAGAAATTCGAGGCGCT
GGTAACGCTCGGCATGGCAAATAGCCGCCTGAAGGATTTCTATGATCTCTGGCTGATTGCCGAGACGTTCGAGCTGGAGC
GGTCCACCCTCGCTGAGGCGGTGCGCCAGACCTTTGCGAGACGAGGGACGGGTATTCCGATGGAGAGGCCAACGGGTCTC
AGCGAAGCCTATGCTGAGGCCTGGGATCGGCAATGGCGGGCGTTCCTTGGTCGCGAACGGATGGCGGCCGCACCACCCGC
CTTGGGCACGGTGGTCGCTGATCTCGGGCGCTTCCTATTGCCGCTGACCCAGCCTGTGGAAGGGGAATGGCGCTGGAAGC
CTTATGAGGGCTGGATTCAGCTGCAAGTAAAGCAGCTTGGATATCCAAAGCGCTGA

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 23159.94 Da
• Isoelectric Point: 9.8640

>AT21705 WP_011654254.1 NC_008381:226551-227171 [Rhizobium leguminosarum bv. viciae 3841]
MTFTTAQRLRALDLLKARGMLRLKDFIAEGIGPETLARLVREEAIVRPARGLYQLPDTQAEAAHALAEAAVLVPRGVVCL
TSALQYHELTLQMPSAVWMAIDRTAWRPKIDYPPIKFVRFKGSALTEGVERHRIEGVEVPITNPARSIVDCFRYRAKVGL
DVAMEGLREGLRRRKTTSDDLWTYAKEARIWSIMRPYVEATVADGA

Nucleotide

• Length: 621 bp

>AT21705 NC_008381:226551-227171 [Rhizobium leguminosarum bv. viciae 3841]
ATGACATTCACCACCGCCCAGAGACTTCGAGCGCTCGACCTCCTGAAAGCGCGGGGGATGCTCCGGCTGAAGGATTTCAT
TGCCGAAGGCATTGGACCGGAGACGCTCGCCCGTCTCGTTCGGGAGGAGGCAATAGTCCGCCCGGCACGAGGCCTGTATC
AACTCCCAGACACTCAGGCTGAGGCGGCCCATGCACTCGCTGAAGCGGCTGTTCTGGTTCCAAGGGGCGTGGTCTGCTTG
ACCTCGGCCCTCCAATACCACGAACTGACGCTGCAGATGCCCTCGGCTGTGTGGATGGCGATCGACCGCACTGCCTGGCG
CCCCAAGATCGACTATCCGCCTATCAAGTTCGTACGCTTCAAAGGGTCCGCTCTCACCGAGGGCGTCGAACGGCATCGCA
TCGAGGGCGTCGAGGTGCCGATCACAAATCCGGCGCGTTCGATCGTCGATTGCTTCCGCTATCGGGCGAAGGTCGGCCTC
GACGTTGCGATGGAGGGGCTGCGCGAGGGGCTCCGCCGGCGCAAGACCACAAGCGACGACCTCTGGACATATGCGAAAGA
GGCCAGGATCTGGTCGATCATGCGACCCTATGTCGAAGCGACGGTGGCCGATGGCGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q1M7T2