Detailed information of TA system TA217046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 764558..765120
• Replicon: chromosome
• Accession: NZ_CP083655
• Organism: Burkholderia vietnamiensis strain FDAARGOS_1488

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1B4LIX1
• Locus tag: LA325_RS13465
• Protein ID: WP_045579168.1
• Coordinates: 764558..764806 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LA325_RS13470
• Protein ID: WP_045579167.1
• Coordinates: 764800..765120 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA325_RS13435 (LA325_13435)	760304..760651	+	348	WP_012367381.1	helix-turn-helix transcriptional regulator	-
LA325_RS13440 (LA325_13440)	760655..761542	+	888	WP_045579173.1	ImmA/IrrE family metallo-endopeptidase	-
LA325_RS13445 (LA325_13445)	761780..762106	+	327	WP_045579172.1	hypothetical protein	-
LA325_RS13450 (LA325_13450)	762349..763140	-	792	WP_045579171.1	SOS response-associated peptidase family protein	-
LA325_RS13455 (LA325_13455)	763271..763597	-	327	WP_045579170.1	H-NS histone family protein	-
LA325_RS13460 (LA325_13460)	763761..764432	+	672	WP_052691567.1	site-specific integrase	-
LA325_RS13465 (LA325_13465)	764558..764806	+	249	WP_045579168.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
LA325_RS13470 (LA325_13470)	764800..765120	+	321	WP_045579167.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
LA325_RS13475 (LA325_13475)	765131..765361	+	231	WP_080938562.1	hypothetical protein	-
LA325_RS13480 (LA325_13480)	765719..765961	+	243	WP_045579166.1	hypothetical protein	-
LA325_RS13485 (LA325_13485)	766932..767174	+	243	WP_126221124.1	hypothetical protein	-
LA325_RS13490 (LA325_13490)	767212..767409	+	198	WP_059889990.1	hypothetical protein	-
LA325_RS13495 (LA325_13495)	767694..767993	+	300	WP_126221125.1	hypothetical protein	-
LA325_RS13500 (LA325_13500)	768007..768453	+	447	WP_126221126.1	hypothetical protein	-
LA325_RS13505 (LA325_13505)	768501..768962	+	462	WP_126221127.1	hypothetical protein	-
LA325_RS13510 (LA325_13510)	769026..769310	+	285	WP_045579163.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	747464..775855	28391

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (9-44)

Antitoxin

• mRNA interferase PemK-like (4-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8979.19 Da
• Isoelectric Point: 4.4929

>T217046 WP_045579168.1 NZ_CP083655:764558-764806 [Burkholderia vietnamiensis]
MKTTIRRMGNSQGVLIPKPVLAQLGLESEVEMEVENDAIVLRRPKHKAREGWAEASKALAESGDDALVMGEFSNADDAEL
AW

Nucleotide

• Length: 249 bp

>T217046 NZ_CP113782:1333301-1333561 [Rothia kristinae]
GTGAAGACGATGACGTATACGGAGTCCCGGGCGCGCTATGCCGAGGTGCTGGACGGCGTGGTGCAGGACCGGGAGGAGGT
GGTGATCACCCGCAGCGGTCACGACCCGGTGGTGATCCTCTCCCTGGCGGAGTATGAGTCGCTGAAGGAGACGGCGCACC
TGCTGCGCTCCCCCTCCAACGCCCGTCGGCTGCTGGATGCGATGCAGCGTTTGGAGGCAGGAGAGGGCGAGCGCCACGAG
CTCGTGGACCCCGATGCCTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11921.20 Da
• Isoelectric Point: 10.5568

>AT217046 WP_045579167.1 NZ_CP083655:764800-765120 [Burkholderia vietnamiensis]
MVMRGEVWLVALDPTLGSEIQKTRPCVVVSPPEMHDHLRTVIVAPMTSKGRPAPFRIPVTFKRKHGLILLDQIRAVDKVR
LVKKEGAIADKTLLDTLRTLQEVFAE

Nucleotide

• Length: 321 bp

>AT217046 NZ_CP113782:1333563-1333823 [Rothia kristinae]
CTGCTGCTGACCTGGGACCCTGACGCCTGGGAGGACTACCTGTGGTGGCAGCGTCAGGATCGCGCCGTGCTACGTCGGAT
CAACACGCTGATCCGGGACATCCAACGCCACGGGAATGAGGGCATCGGCAAACCGGAGCCGCTTCGTCATGACTTCGCCG
GATACTGGTCCCGCCGGATCACTGCGGAACACCGACTGGTGTATCGACTCACCGACCGCCAGGTGCTCATCGCCGCGTGC
CGCTATCACTACGGGCAGTGA

Structures

Toxin

• AlphaFold DB: A0A1B4LIX1