Detailed information of TA system TA217042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 96128..96650
• Replicon: plasmid unnamed1
• Accession: NZ_CP083652
• Organism: Citrobacter amalonaticus strain FDAARGOS_1489

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: LA348_RS23205
• Protein ID: WP_141227313.1
• Coordinates: 96128..96412 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A376C2P3
• Locus tag: LA348_RS23210
• Protein ID: WP_048030981.1
• Coordinates: 96402..96650 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA348_RS23185 (LA348_23185)	91475..92446	-	972	WP_212807616.1	plasmid segregation protein ParM	-
LA348_RS23190 (LA348_23190)	92678..93109	+	432	WP_040078803.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LA348_RS23195 (LA348_23195)	93109..94380	+	1272	WP_141227314.1	Y-family DNA polymerase	-
LA348_RS23200 (LA348_23200)	94770..96112	+	1343	Protein_111	ISNCY family transposase	-
LA348_RS23205 (LA348_23205)	96128..96412	-	285	WP_141227313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LA348_RS23210 (LA348_23210)	96402..96650	-	249	WP_048030981.1	plasmid stabilization protein	Antitoxin
LA348_RS23215 (LA348_23215)	97110..97904	-	795	WP_141227312.1	helix-turn-helix transcriptional regulator	-
LA348_RS23220 (LA348_23220)	97990..99180	+	1191	WP_141227311.1	MFS transporter	-
LA348_RS23225 (LA348_23225)	99253..99834	+	582	WP_141227310.1	NAD(P)H-dependent oxidoreductase	-
LA348_RS23230 (LA348_23230)	99960..100865	-	906	WP_141227309.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..185823	185823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11031.77 Da
• Isoelectric Point: 10.5388

>T217042 WP_141227313.1 NZ_CP083652:c96412-96128 [Citrobacter amalonaticus]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNVTLNRN

Nucleotide

• Length: 285 bp

>T217042 NZ_CP113541:c4131429-4131145 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9039.25 Da
• Isoelectric Point: 3.9947

>AT217042 WP_048030981.1 NZ_CP083652:c96650-96402 [Citrobacter amalonaticus]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGERVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT217042 NZ_CP113541:c4131661-4131419 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A376C2P3