Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 2429294..2429892 Replicon chromosome
Accession NZ_CP083648
Organism Corynebacterium coyleae strain FDAARGOS_1492

Toxin (Protein)


Gene name PumA Uniprot ID A0A2I1V9Y7
Locus tag LA324_RS11565 Protein ID WP_070482101.1
Coordinates 2429294..2429602 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A1F1YMH8
Locus tag LA324_RS11570 Protein ID WP_070362126.1
Coordinates 2429599..2429892 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA324_RS11555 (LA324_11555) 2425279..2426397 + 1119 Protein_2245 IS256 family transposase -
LA324_RS11560 (LA324_11560) 2426819..2428987 + 2169 WP_224213107.1 hypothetical protein -
LA324_RS12425 2428999..2429130 - 132 WP_263436524.1 hypothetical protein -
LA324_RS11565 (LA324_11565) 2429294..2429602 + 309 WP_070482101.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LA324_RS11570 (LA324_11570) 2429599..2429892 + 294 WP_070362126.1 putative addiction module antidote protein Antitoxin
LA324_RS11575 (LA324_11575) 2430105..2431016 - 912 Protein_2250 IS3 family transposase -
LA324_RS11580 (LA324_11580) 2431013..2431267 - 255 WP_244268753.1 transposase -
LA324_RS11585 (LA324_11585) 2431378..2431536 - 159 Protein_2252 IS3 family transposase -
LA324_RS11590 (LA324_11590) 2432457..2433429 + 973 Protein_2253 IS256 family transposase -
LA324_RS11595 (LA324_11595) 2433484..2433801 + 318 WP_013887466.1 transposase -
LA324_RS11600 (LA324_11600) 2433859..2434188 + 330 WP_224213108.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2422135..2442372 20237
- flank IS/Tn - - 2425375..2426397 1022


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(2-96)

Antitoxin

Uncharacterized protein HI1420

(15-94)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11554.24 Da        Isoelectric Point: 9.4826

>T217024 WP_070482101.1 NZ_CP083648:2429294-2429602 [Corynebacterium coyleae]
VEIISSSLFDAWLGELKDKHAQRRILYAIARCEAHGRMIGDIKPVGEKVSEMRFHFGPGYRVYYTRLGAITVFLLAGGDK
SSQDKDIRTAQKLAQQVREEQS

Download         Length: 309 bp

>T217024 NZ_CP113540:3636527-3636784 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10578.05 Da        Isoelectric Point: 7.0095

>AT217024 WP_070362126.1 NZ_CP083648:2429599-2429892 [Corynebacterium coyleae]
VKEVTFSAFDASKYLDSQEAVNDYLAIALEDGDTKTVQVVLRDIARAHGMSQLAKETQLNRESLYKSLSKDGNPSFAAIT
KIMKALGLKITLATQNA

Download         Length: 294 bp

>AT217024 NZ_CP113540:c3636702-3636370 [Salmonella enterica]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I1V9Y7

Antitoxin

Source ID Structure
AlphaFold DB A0A1F1YMH8

References