Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 17951..19574 Replicon plasmid unnamed
Accession NZ_CP083644
Organism Enterobacter bugandensis strain FDAARGOS_1496

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LA326_RS22575 Protein ID WP_000088044.1
Coordinates 17951..19276 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LA326_RS22580 Protein ID WP_012477377.1
Coordinates 19281..19574 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA326_RS22545 (LA326_22545) 13937..14794 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
LA326_RS22550 (LA326_22550) 14810..15118 - 309 WP_001224686.1 hypothetical protein -
LA326_RS22555 (LA326_22555) 15667..16092 - 426 WP_000278471.1 hypothetical protein -
LA326_RS22560 (LA326_22560) 16345..17160 - 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
LA326_RS22565 (LA326_22565) 17173..17583 - 411 WP_000985909.1 hypothetical protein -
LA326_RS22570 (LA326_22570) 17684..17890 - 207 WP_000134171.1 hypothetical protein -
LA326_RS22575 (LA326_22575) 17951..19276 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LA326_RS22580 (LA326_22580) 19281..19574 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LA326_RS22585 (LA326_22585) 19975..21363 - 1389 WP_000083579.1 hypothetical protein -
LA326_RS22590 (LA326_22590) 21368..21865 - 498 WP_000340139.1 membrane protein -
LA326_RS22595 (LA326_22595) 22068..22724 - 657 WP_000462754.1 trypsin-like serine protease -
LA326_RS22600 (LA326_22600) 23383..23775 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / catA1 / ant(3'')-Ia / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / qnrB1 / dfrA14 - 1..298988 298988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T217019 WP_000088044.1 NZ_CP083644:c19276-17951 [Enterobacter bugandensis]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T217019 NZ_CP113540:836177-836575 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT217019 WP_012477377.1 NZ_CP083644:c19574-19281 [Enterobacter bugandensis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT217019 NZ_CP113540:835950-836177 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References