Detailed information of TA system TA217016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3400176..3400796
• Replicon: chromosome
• Accession: NZ_CP083643
• Organism: Enterobacter bugandensis strain FDAARGOS_1496

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: LA326_RS16235
• Protein ID: WP_008499287.1
• Coordinates: 3400578..3400796 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8E1RNX1
• Locus tag: LA326_RS16230
• Protein ID: WP_028012188.1
• Coordinates: 3400176..3400550 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA326_RS16220 (LA326_16220)	3395304..3396497	+	1194	WP_028012190.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
LA326_RS16225 (LA326_16225)	3396520..3399666	+	3147	WP_047367620.1	multidrug efflux RND transporter permease subunit AcrB	-
LA326_RS16230 (LA326_16230)	3400176..3400550	+	375	WP_028012188.1	Hha toxicity modulator TomB	Antitoxin
LA326_RS16235 (LA326_16235)	3400578..3400796	+	219	WP_008499287.1	HHA domain-containing protein	Toxin
LA326_RS16240 (LA326_16240)	3401006..3401557	+	552	WP_028012187.1	maltose O-acetyltransferase	-
LA326_RS16245 (LA326_16245)	3401674..3402141	+	468	WP_028012186.1	YlaC family protein	-
LA326_RS16250 (LA326_16250)	3402113..3403573	-	1461	WP_088205222.1	PLP-dependent aminotransferase family protein	-
LA326_RS16255 (LA326_16255)	3403675..3404385	+	711	WP_088205223.1	GNAT family protein	-
LA326_RS16260 (LA326_16260)	3404382..3404522	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
LA326_RS16265 (LA326_16265)	3404525..3404785	-	261	WP_006176933.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T217016 WP_008499287.1 NZ_CP083643:3400578-3400796 [Enterobacter bugandensis]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T217016 NZ_CP113540:310524-310892 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.30 Da
• Isoelectric Point: 4.8886

>AT217016 WP_028012188.1 NZ_CP083643:3400176-3400550 [Enterobacter bugandensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT217016 NZ_CP113540:310306-310527 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38