Detailed information of TA system TA021701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 726784..727319
• Replicon: plasmid pRL12
• Accession: NC_008378
• Organism: Rhizobium leguminosarum bv. viciae 3841

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8G2MTM4
• Locus tag: RL_RS36295
• Protein ID: WP_028742965.1
• Coordinates: 727044..727319 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8G2J5T9
• Locus tag: RL_RS36290
• Protein ID: WP_028742964.1
• Coordinates: 726784..727044 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RL_RS36270	722613..723626	-	1014	WP_011649425.1	ABC transporter substrate-binding protein	-
RL_RS36275	723971..724981	-	1011	WP_011649426.1	helix-turn-helix domain-containing protein	-
RL_RS36280	724978..725556	-	579	WP_011649427.1	isochorismatase family protein	-
RL_RS36285	725750..726499	-	750	WP_011649428.1	FadR family transcriptional regulator	-
RL_RS36290	726784..727044	+	261	WP_028742964.1	ribbon-helix-helix domain-containing protein	Antitoxin
RL_RS36295	727044..727319	+	276	WP_028742965.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RL_RS36300	727326..728333	-	1008	WP_011649431.1	LacI family transcriptional regulator	-
RL_RS36305	728541..729863	+	1323	WP_011649432.1	alpha-L-fucosidase	-
RL_RS36310	729914..731137	+	1224	WP_011649433.1	sugar ABC transporter substrate-binding protein	-
RL_RS36315	731220..732194	+	975	WP_173364467.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..870021	870021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10826.48 Da
• Isoelectric Point: 9.9288

>T21701 WP_028742965.1 NC_008378:727044-727319 [Rhizobium leguminosarum bv. viciae 3841]
MELKWTSKAVSDVARLYDFLAPVDRLAAARTVQALTKAPARLLDQPRLGERLEEFDPREVRRILVGRYELRYEIQQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T21701 NC_008378:727044-727319 [Rhizobium leguminosarum bv. viciae 3841]
ATGGAACTCAAATGGACGAGCAAAGCGGTCTCCGACGTTGCCCGCTTGTACGACTTTCTCGCTCCTGTAGACCGCCTCGC
GGCGGCTCGAACCGTCCAGGCACTGACGAAGGCACCTGCGCGGCTTCTTGATCAGCCGCGCCTCGGCGAGCGGCTTGAGG
AGTTCGATCCTCGGGAGGTCCGCCGCATTCTCGTCGGCCGTTACGAGCTGCGCTATGAAATCCAGCAGTCGACGATCTAT
GTGTTGCGGCTTTGGCATACGCGTGAGGACCGATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9630.82 Da
• Isoelectric Point: 4.6005

>AT21701 WP_028742964.1 NC_008378:726784-727044 [Rhizobium leguminosarum bv. viciae 3841]
METKVLTAHVPLSLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDDGNVVDHQSVQAWADSLDGDA
PISLPR

Nucleotide

• Length: 261 bp

>AT21701 NC_008378:726784-727044 [Rhizobium leguminosarum bv. viciae 3841]
ATGGAGACCAAGGTTCTGACCGCCCATGTTCCGCTGTCGCTTGCGCAGAAGGTCGATCAACTCGCGTCGCGGCTCGAACG
CTCGCGTGGCTGGATCGTCAAGCAGGCCCTGACGGCATGGATCGACCAGGAGGAGGAGCGCCGGCGTTTGACGCTTGAGG
CGTTGGCCGACGTCGATGACGGTAACGTTGTCGATCACCAGTCTGTCCAAGCCTGGGCCGATAGTCTTGACGGCGACGCG
CCGATTTCGTTGCCTCGGTGA