217004

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 246852..248475 Replicon plasmid unnamed
Accession NZ_CP083642
Organism Enterobacter bugandensis strain FDAARGOS_1497

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LA318_RS23765 Protein ID WP_000088044.1
Coordinates 247150..248475 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LA318_RS23760 Protein ID WP_012477377.1
Coordinates 246852..247145 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA318_RS23740 (LA318_23740) 242651..243043 + 393 WP_000341066.1 hypothetical protein -
LA318_RS23745 (LA318_23745) 243702..244358 + 657 WP_000462754.1 trypsin-like serine protease -
LA318_RS23750 (LA318_23750) 244561..245058 + 498 WP_000340139.1 membrane protein -
LA318_RS23755 (LA318_23755) 245063..246451 + 1389 WP_000083579.1 hypothetical protein -
LA318_RS23760 (LA318_23760) 246852..247145 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LA318_RS23765 (LA318_23765) 247150..248475 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LA318_RS23770 (LA318_23770) 248536..248742 + 207 WP_000134171.1 hypothetical protein -
LA318_RS23775 (LA318_23775) 248843..249253 + 411 WP_000985909.1 hypothetical protein -
LA318_RS23780 (LA318_23780) 249266..250081 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
LA318_RS23785 (LA318_23785) 250334..250759 + 426 WP_000278471.1 hypothetical protein -
LA318_RS23790 (LA318_23790) 251308..251616 + 309 WP_001224686.1 hypothetical protein -
LA318_RS23795 (LA318_23795) 251632..252489 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / ant(3'')-Ia / catA1 / tet(A) / dfrA14 / qnrB1 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 - 1..298988 298988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T217004 WP_000088044.1 NZ_CP083642:247150-248475 [Enterobacter bugandensis]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T217004 NZ_CP113539:843316-843729 [Salmonella enterica]
GTGATCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT217004 WP_012477377.1 NZ_CP083642:246852-247145 [Enterobacter bugandensis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT217004 NZ_CP113539:843069-843335 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGATCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References