Detailed information of TA system TA021700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 393394..394061
• Replicon: plasmid pRL12
• Accession: NC_008378
• Organism: Rhizobium leguminosarum bv. viciae 3841

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q1M497
• Locus tag: RL_RS34745
• Protein ID: WP_011649135.1
• Coordinates: 393394..393804 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1M496
• Locus tag: RL_RS34750
• Protein ID: WP_011649136.1
• Coordinates: 393801..394061 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RL_RS34730	389251..390072	+	822	WP_011649132.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
RL_RS34735	390094..391008	-	915	WP_011649133.1	hydrogen peroxide-inducible genes activator	-
RL_RS34740	391152..393332	+	2181	WP_011649134.1	catalase/peroxidase HPI	-
RL_RS34745	393394..393804	-	411	WP_011649135.1	type II toxin-antitoxin system VapC family toxin	Toxin
RL_RS34750	393801..394061	-	261	WP_011649136.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RL_RS34755	394197..394754	-	558	WP_028742374.1	DUF3299 domain-containing protein	-
RL_RS34760	394862..395830	-	969	WP_041936998.1	zinc-dependent alcohol dehydrogenase family protein	-
RL_RS34765	395884..396300	+	417	WP_011649139.1	acyl-CoA thioesterase	-
RL_RS34770	396330..397859	+	1530	WP_041936999.1	AMP-binding protein	-
RL_RS34775	397837..399042	+	1206	WP_011649141.1	thiolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..870021	870021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-45)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14781.89 Da
• Isoelectric Point: 4.9105

>T21700 WP_011649135.1 NC_008378:c393804-393394 [Rhizobium leguminosarum bv. viciae 3841]
VITHLIDTNAVIALIGRKSETLLKRVIDSDEGSIGLSTVVMHELYYGAYKSTKISYNLETLRLFVADFSVVGFEQEDALA
AGEIRAALAAKGTPIGPYDVLIAAQARTRDLVLVTNNVGEFGRVDGLRVEDWTVGR

Nucleotide

• Length: 411 bp

>T21700 NC_008378:c393804-393394 [Rhizobium leguminosarum bv. viciae 3841]
GTGATCACGCATCTTATCGACACCAATGCGGTGATCGCCCTGATCGGCAGGAAATCCGAAACACTGCTGAAGCGCGTGAT
CGATAGCGACGAGGGATCGATCGGGCTGTCGACCGTGGTGATGCACGAGCTTTATTATGGCGCCTATAAAAGCACGAAAA
TCTCCTACAATCTCGAAACCCTGCGGCTGTTCGTGGCGGATTTCTCAGTCGTCGGTTTCGAGCAGGAGGATGCGTTGGCC
GCTGGGGAGATCCGTGCCGCATTGGCGGCAAAGGGAACGCCGATCGGCCCCTATGATGTTCTGATCGCCGCTCAGGCCAG
GACCCGGGATCTGGTTCTCGTCACCAACAATGTCGGAGAATTCGGCCGCGTCGATGGTCTGCGTGTCGAGGACTGGACGG
TCGGGCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9948.12 Da
• Isoelectric Point: 5.1532

>AT21700 WP_011649136.1 NC_008378:c394061-393801 [Rhizobium leguminosarum bv. viciae 3841]
MPQYARVFQSGNSQAVRLPKEFRFDVDQVEVTREGDAVILRPRADRGMRWASLHSALERGLSDDFMAEGREQPEAQERPD
LQNLFK

Nucleotide

• Length: 261 bp

>AT21700 NC_008378:c394061-393801 [Rhizobium leguminosarum bv. viciae 3841]
ATGCCGCAATACGCTCGCGTTTTCCAATCCGGCAATTCGCAAGCCGTCCGGCTGCCGAAAGAGTTCCGCTTCGACGTCGA
CCAGGTGGAGGTGACGCGGGAAGGCGATGCTGTGATCCTTCGGCCACGGGCCGATCGCGGTATGCGGTGGGCATCGTTGC
ATAGCGCCTTGGAGCGCGGCCTCAGCGACGATTTCATGGCGGAAGGGCGAGAGCAGCCGGAGGCGCAGGAGAGGCCTGAT
CTTCAGAACCTGTTCAAGTGA

Structures

Toxin

• AlphaFold DB: Q1M497

Antitoxin

• AlphaFold DB: Q1M496