Detailed information of TA system TA216993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1538602..1539399
• Replicon: chromosome
• Accession: NZ_CP083641
• Organism: Enterobacter bugandensis strain FDAARGOS_1497

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: LA318_RS07265
• Protein ID: WP_172616463.1
• Coordinates: 1538899..1539399 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: LA318_RS07260
• Protein ID: WP_008499829.1
• Coordinates: 1538602..1538871 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA318_RS07230 (LA318_07230)	1534232..1534675	-	444	WP_028012647.1	helix-turn-helix domain-containing protein	-
LA318_RS07235 (LA318_07235)	1534828..1536069	+	1242	WP_088204980.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
LA318_RS07240 (LA318_07240)	1536103..1536330	-	228	WP_028012649.1	YccJ family protein	-
LA318_RS07245 (LA318_07245)	1536349..1536945	-	597	WP_041910554.1	NAD(P)H:quinone oxidoreductase	-
LA318_RS07250 (LA318_07250)	1537336..1537506	+	171	WP_001273664.1	general stress protein	-
LA318_RS07255 (LA318_07255)	1537623..1538540	+	918	WP_088204981.1	DMT family transporter	-
LA318_RS07260 (LA318_07260)	1538602..1538871	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
LA318_RS07265 (LA318_07265)	1538899..1539399	+	501	WP_172616463.1	GNAT family N-acetyltransferase	Toxin
LA318_RS07270 (LA318_07270)	1539458..1540780	-	1323	WP_048959639.1	pyrimidine utilization transport protein G	-
LA318_RS07275 (LA318_07275)	1540802..1541296	-	495	WP_028012654.1	pyrimidine utilization flavin reductase protein F	-
LA318_RS07280 (LA318_07280)	1541306..1541896	-	591	WP_041910551.1	malonic semialdehyde reductase	-
LA318_RS07285 (LA318_07285)	1541906..1542706	-	801	WP_028012656.1	pyrimidine utilization protein D	-
LA318_RS07290 (LA318_07290)	1542714..1543100	-	387	WP_088204983.1	pyrimidine utilization protein C	-
LA318_RS07295 (LA318_07295)	1543112..1543801	-	690	WP_028012657.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18873.63 Da
• Isoelectric Point: 8.4785

>T216993 WP_172616463.1 NZ_CP083641:1538899-1539399 [Enterobacter bugandensis]
MFSEGTDYDFGDFDCGEPSLNAFLREHLVRQHNGRILRAYLLKERDHPRVLGYYTLSGSCFERAMLPSKTQQRRIPYLNV
PSVTLGRLAIHKELQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALSEQAKRFYLKLGFIPLAGENSHSLFFPTKAIE
RLFEQA

Nucleotide

• Length: 501 bp

>T216993 NZ_CP113538:2043743-2043846 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT216993 WP_008499829.1 NZ_CP083641:1538602-1538871 [Enterobacter bugandensis]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT216993 NZ_CP113538:c2043848-2043703 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Antitoxin

• AlphaFold DB: W7PG43