Detailed information of TA system TA216991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 947340..947960
• Replicon: chromosome
• Accession: NZ_CP083641
• Organism: Enterobacter bugandensis strain FDAARGOS_1497

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: LA318_RS04460
• Protein ID: WP_008499287.1
• Coordinates: 947340..947558 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8E1RNX1
• Locus tag: LA318_RS04465
• Protein ID: WP_028012188.1
• Coordinates: 947586..947960 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA318_RS04430 (LA318_04430)	943351..943611	+	261	WP_006176933.1	type B 50S ribosomal protein L31	-
LA318_RS04435 (LA318_04435)	943614..943754	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
LA318_RS04440 (LA318_04440)	943751..944461	-	711	WP_088205223.1	GNAT family protein	-
LA318_RS04445 (LA318_04445)	944563..946023	+	1461	WP_088205222.1	PLP-dependent aminotransferase family protein	-
LA318_RS04450 (LA318_04450)	945995..946462	-	468	WP_028012186.1	YlaC family protein	-
LA318_RS04455 (LA318_04455)	946579..947130	-	552	WP_028012187.1	maltose O-acetyltransferase	-
LA318_RS04460 (LA318_04460)	947340..947558	-	219	WP_008499287.1	HHA domain-containing protein	Toxin
LA318_RS04465 (LA318_04465)	947586..947960	-	375	WP_028012188.1	Hha toxicity modulator TomB	Antitoxin
LA318_RS04470 (LA318_04470)	948470..951616	-	3147	WP_047367620.1	multidrug efflux RND transporter permease subunit AcrB	-
LA318_RS04475 (LA318_04475)	951639..952832	-	1194	WP_028012190.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T216991 WP_008499287.1 NZ_CP083641:c947558-947340 [Enterobacter bugandensis]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T216991 NZ_CP113538:879197-879595 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.30 Da
• Isoelectric Point: 4.8886

>AT216991 WP_028012188.1 NZ_CP083641:c947960-947586 [Enterobacter bugandensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT216991 NZ_CP113538:878970-879197 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38