Detailed information of TA system TA021699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 134847..135511
• Replicon: plasmid pRL12
• Accession: NC_008378
• Organism: Rhizobium leguminosarum bv. viciae 3841

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q1M4X2
• Locus tag: RL_RS33600
• Protein ID: WP_011648925.1
• Coordinates: 134847..135260 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RL_RS33605
• Protein ID: WP_028742527.1
• Coordinates: 135257..135511 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RL_RS33585	130459..131472	-	1014	WP_011648922.1	ABC transporter permease	-
RL_RS33590	131542..133212	-	1671	WP_028742529.1	ABC transporter substrate-binding protein	-
RL_RS33595	133407..134417	-	1011	WP_041936980.1	GlxA family transcriptional regulator	-
RL_RS33600	134847..135260	-	414	WP_011648925.1	type II toxin-antitoxin system VapC family toxin	Toxin
RL_RS33605	135257..135511	-	255	WP_028742527.1	plasmid stabilization protein	Antitoxin
RL_RS33610	135737..136705	-	969	WP_041936981.1	cyclase family protein	-
RL_RS33615	136751..138124	-	1374	WP_011648928.1	amidase	-
RL_RS33620	138144..139352	-	1209	WP_011648929.1	extracellular solute-binding protein	-
RL_RS33625	139349..140389	-	1041	WP_011648930.1	GTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..870021	870021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14804.20 Da
• Isoelectric Point: 5.7251

>T21699 WP_011648925.1 NC_008378:c135260-134847 [Rhizobium leguminosarum bv. viciae 3841]
MILLDTNVISEPWKPIPDGAVVAWLDAQAIETLFVSAITIAELRFAIAAMPAGKRQTILRDRLEEEVLPHFAERILPFDL
AASQFYSELMARARVSGKGSARGHIAATAVANGLAVATRDTGPFEAVGLKVINPWLP

Nucleotide

• Length: 414 bp

>T21699 NC_008378:c135260-134847 [Rhizobium leguminosarum bv. viciae 3841]
ATGATCCTGTTGGACACCAACGTGATATCGGAGCCGTGGAAACCGATTCCTGACGGAGCAGTGGTCGCTTGGTTGGACGC
CCAAGCCATCGAAACTCTCTTCGTTTCCGCAATAACAATTGCTGAACTACGTTTTGCGATCGCTGCCATGCCCGCAGGCA
AACGGCAAACTATCCTTCGCGATCGCCTGGAGGAAGAAGTGCTTCCGCATTTTGCCGAACGCATCCTGCCTTTCGATCTT
GCCGCCAGCCAATTTTACTCGGAACTGATGGCGCGCGCTCGAGTATCCGGGAAAGGATCAGCAAGGGGACATATCGCAGC
CACCGCCGTAGCGAACGGCCTTGCCGTCGCAACTCGCGACACAGGCCCTTTTGAGGCCGTTGGGTTGAAAGTGATTAACC
CCTGGCTCCCATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9256.41 Da
• Isoelectric Point: 6.7270

>AT21699 WP_028742527.1 NC_008378:c135511-135257 [Rhizobium leguminosarum bv. viciae 3841]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDIIEAAVRPSERVKLGSLLAAIGRDAELSNSDVEALQQNRNKAPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT21699 NC_008378:c135511-135257 [Rhizobium leguminosarum bv. viciae 3841]
ATGCCGGCGGTCACCATTCGCAATCTTTCTGACGAAACACATCGCGCCCTACGGGTGCGCGCAGCTCATCATGGCCGAAG
CACAGAGGCCGAAATCCGTGACATTATCGAGGCCGCCGTGCGTCCATCTGAACGCGTGAAGCTCGGCTCCTTACTTGCGG
CCATCGGTCGTGACGCTGAGCTCTCCAACAGCGACGTTGAAGCCTTGCAGCAAAATCGCAATAAGGCGCCTGCTGAACCC
ATGACTTTCGAATGA

Structures

Toxin

• AlphaFold DB: Q1M4X2