Detailed information of TA system TA216951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 101425..102161
• Replicon: plasmid unnamed1
• Accession: NZ_CP083634
• Organism: Klebsiella quasipneumoniae strain FDAARGOS_1503

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: LA349_RS26120
• Protein ID: WP_004187044.1
• Coordinates: 101425..101907 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LA349_RS26125
• Protein ID: WP_003026799.1
• Coordinates: 101895..102161 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA349_RS26095 (LA349_26095)	97324..97602	+	279	WP_004197671.1	hypothetical protein	-
LA349_RS26100 (LA349_26100)	97630..97821	-	192	Protein_108	hypothetical protein	-
LA349_RS26105 (LA349_26105)	97842..99239	-	1398	WP_009653207.1	ISNCY family transposase	-
LA349_RS26110 (LA349_26110)	99428..99823	-	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
LA349_RS26115 (LA349_26115)	99872..101218	-	1347	WP_077253535.1	ISNCY family transposase	-
LA349_RS26120 (LA349_26120)	101425..101907	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
LA349_RS26125 (LA349_26125)	101895..102161	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LA349_RS26130 (LA349_26130)	102312..103016	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LA349_RS26135 (LA349_26135)	103151..103220	-	70	Protein_115	hypothetical protein	-
LA349_RS26140 (LA349_26140)	103357..103878	+	522	WP_004206587.1	RNA polymerase sigma factor FecI	-
LA349_RS26145 (LA349_26145)	103875..104828	+	954	WP_004206586.1	ferric citrate uptake sigma factor regulator FecR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia	-	1..151102	151102
	-	inside	IScluster/Tn	-	-	97842..103016	5174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T216951 WP_004187044.1 NZ_CP083634:c101907-101425 [Klebsiella quasipneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T216951 NZ_CP113535:c1109260-1108733 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT216951 WP_003026799.1 NZ_CP083634:c102161-101895 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT216951 NZ_CP113535:c1109547-1109257 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66