Detailed information of TA system TA216923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3283633..3284276
• Replicon: chromosome
• Accession: NZ_CP083630
• Organism: Leclercia adecarboxylata strain FDAARGOS_1505

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LA332_RS15715
• Protein ID: WP_015387203.1
• Coordinates: 3283860..3284276 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3E1ZVJ5
• Locus tag: LA332_RS15710
• Protein ID: WP_032616516.1
• Coordinates: 3283633..3283863 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA332_RS15690 (LA332_15690)	3279826..3280917	+	1092	WP_032616520.1	3-isopropylmalate dehydrogenase	-
LA332_RS15695 (LA332_15695)	3280920..3282320	+	1401	WP_032616519.1	3-isopropylmalate dehydratase large subunit	-
LA332_RS15700 (LA332_15700)	3282331..3282936	+	606	WP_032616518.1	3-isopropylmalate dehydratase small subunit	-
LA332_RS15705 (LA332_15705)	3283028..3283180	-	153	WP_032616517.1	Hok/Gef family protein	-
LA332_RS15710 (LA332_15710)	3283633..3283863	+	231	WP_032616516.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LA332_RS15715 (LA332_15715)	3283860..3284276	+	417	WP_015387203.1	type II toxin-antitoxin system VapC family toxin	Toxin
LA332_RS15720 (LA332_15720)	3284349..3284636	-	288	WP_032610795.1	hypothetical protein	-
LA332_RS15725 (LA332_15725)	3284889..3285842	-	954	WP_032616508.1	LysR family transcriptional regulator	-
LA332_RS15730 (LA332_15730)	3285994..3286980	+	987	WP_032616507.1	aldo/keto reductase	-
LA332_RS15735 (LA332_15735)	3287359..3288558	+	1200	WP_032616506.1	multidrug effflux MFS transporter	-
LA332_RS15740 (LA332_15740)	3288536..3288769	-	234	Protein_3072	alanine racemase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3283028..3307903	24875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14924.31 Da
• Isoelectric Point: 7.2449

>T216923 WP_015387203.1 NZ_CP083630:3283860-3284276 [Leclercia adecarboxylata]
VIKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAN

Nucleotide

• Length: 417 bp

>T216923 NZ_CP113519:c3982047-3981478 [Pseudomonas sp. GOM7]
ATGTTCAAACCGCAATTGGTCACCTTGCAACGGGGTGCGCTGCGCCTGGAGCCGTTGGCCGATAACGACATCCCGGCATT
GGTGGCGCTGGCCGAGGCCAACCGTGAGGAGCTGCTGTACATGAATGGCACCCTGCGCCTGGACTGGTACCGCAGTGCGC
TGGCCGACCAGCGTGAACAACGGGCGCTGCCGTTCGTCATCCGCTTGGGCGATCGTCTAGTGGGTACCACGCGCTTCGCC
GATTTCCTCAATGCACTGCCGGCTTGCGAGATCGGCTGGACCTGGCTCGATCGTGAGCAGCATGGCAGTGGCCTGAATAC
CACCATCAAGTACCTGATGCTCAAGCATGCTTTCGACAACTGGGGCATGGTGCGTGTGCAGCTCAAGACCGCCGCCAGCA
ACCTGCGCTCGCAGCGCGCCATCGAAAAGCTCGGTGCCGTGCGCGAAGGCTTGCTGCGCAACCATCGGCGCCTGGCCGAC
GGCCGCCTCGACGATACGGTGCTGTACAGCATCACCGACCAGGAATGGCCGCAGGTGCGTCAGGCATTGGAAGCGAGCTT
CGGCGCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8687.72 Da
• Isoelectric Point: 4.4563

>AT216923 WP_032616516.1 NZ_CP083630:3283633-3283863 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFTQLEKADSDFMSERGDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT216923 NZ_CP113519:c3981474-3980641 [Pseudomonas sp. GOM7]
ATGCAACCGGATGCAACGAGGGAGCACGTACGTTTCAGCACCAGCGCGGATGCGCAGGGGGTCGAGCTGTTGTCCGCGCG
TTTCATCGAGCACCGTTTCGCCCCTCACGTGCACGATGGGCATGTGATTGCGGTGCTCGAGGCGGGCGCCGAGCGTTATC
GCTATCGTGGTATCGAGCACCTGGCGGGGGCCGGTTGCATCGCTTTGCTCAACCCGGATGAGGTGCACACAGGCTCCAAG
GGGGCCGAGCAGGGATGGCAGTATCGGGTGTTCTATCCGCAGCCGGGCACCTTCGACACCCTGCTCGAGGAGTTGGAGCT
GCGGCCGAGCACGCCGATGTTTCAGGACAGCGTGCACCATGATCCGCTGCTGGCTACACGGCTGAGCGTTCTGCACCGCC
TGCTGGAGACTCCGCGCAGTGATGCCTTGCAACGGCAGACCCTGTGGCGTGAGGTGATGCTCGAACTTTTGCAGCGCTAT
GCGCGCCTGCCGGTGCCGCGCGCAGCCGGGCGCGAGCCACGGGTAGTGGCCATGGCCAAGGAGCTTCTGGCCAGTCGGTT
GTACGAGCCGCCGTCACTGGAGATGCTGGCGGAGAGCGTCAATCTCTCGCCTTTTCATTTCGCCCGGGTCTTTCGTCGCG
CCACCGGCTTGCCACCCCATGCCTGGCTCAAGCAGCGACGCCTGGAGCAGGCTAGGGCCCTGTTGCGACAGGGCAGTGCA
CCGCTGTCGGTAGCGATGCAACTGGGCTTTGCCGACCAGAGCCACCTGTCGCGCCAGTTCAAGCAGGCCTACGGTATTGC
CCCCGGCGAATATCGACGGGCTTGCCAGACCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3E1ZVJ5