Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2944994..2945613 Replicon chromosome
Accession NZ_CP083630
Organism Leclercia adecarboxylata strain FDAARGOS_1505

Toxin (Protein)


Gene name Hha Uniprot ID A0A345CDT0
Locus tag LA332_RS14135 Protein ID WP_032616864.1
Coordinates 2945395..2945613 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3E1ZZ82
Locus tag LA332_RS14130 Protein ID WP_032616865.1
Coordinates 2944994..2945386 (+) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA332_RS14120 (LA332_14120) 2940146..2941339 + 1194 WP_032616867.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LA332_RS14125 (LA332_14125) 2941362..2944502 + 3141 WP_032616866.1 multidrug efflux RND transporter permease subunit AcrB -
LA332_RS14130 (LA332_14130) 2944994..2945386 + 393 WP_032616865.1 Hha toxicity modulator TomB Antitoxin
LA332_RS14135 (LA332_14135) 2945395..2945613 + 219 WP_032616864.1 HHA domain-containing protein Toxin
LA332_RS14140 (LA332_14140) 2945885..2946349 + 465 WP_032616863.1 YlaC family protein -
LA332_RS14145 (LA332_14145) 2946409..2946549 - 141 WP_032616862.1 type B 50S ribosomal protein L36 -
LA332_RS14150 (LA332_14150) 2946815..2948383 + 1569 WP_032616861.1 EAL domain-containing protein -
LA332_RS14155 (LA332_14155) 2948416..2948769 - 354 WP_032616860.1 DUF1428 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T216922 WP_032616864.1 NZ_CP083630:2945395-2945613 [Leclercia adecarboxylata]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Download         Length: 219 bp

>T216922 NZ_CP113519:c1898268-1897990 [Pseudomonas sp. GOM7]
ATGATCAAATCTTTCCAGCACAAAGGGCTGCGCAAGCTATTTGAAACGGGCAGCACAGCAGGCGTACAACCCGCCCATGC
CAAGCGGCTACGAATGCAGCTAGCAGCCCTGGATACAGCCATGACGGTAGACGACATGGACATCCCTGGCTTCCGCCTGC
ACCCGCTCAAGGGAGAAATGCGTGGGCGCTGGTCGATCATGGTGAACGGCAACTGGCGGCTGACGTTCGAGTTTCGCGAC
GGAAACGCTTATGTCCTGGACTATGAGGATTACCACTGA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 15093.98 Da        Isoelectric Point: 4.8941

>AT216922 WP_032616865.1 NZ_CP083630:2944994-2945386 [Leclercia adecarboxylata]
MDEYSPKRQDIAQLKFLCESLYHDCLSNLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNASKANPLASPVKIITNN

Download         Length: 393 bp

>AT216922 NZ_CP113519:c1897990-1897703 [Pseudomonas sp. GOM7]
ATGAACATGCACAACCCGCCCCATCCTGGTGAATTCATCACCGATATCTACCTGGAGCCGAACGGCATCAGCGGTCGTGA
GCTGGCCGAAAAGCTGCGCGTAGCACCCTCGACCCTGAGCCGTGTGCTCAAGGGCACCAGCCGCGTAACGCCGGAAATGG
CTCTGCGCCTCTCGGTAGCACTTGGTCGCTCGCCTGAGAGCTGGCTAGCTATGCAAGATGCTTACGACTTATGGAATGCC
CGTCAGCATGTAGATCTGCAGCAGGTTGGAAAGCTGGCGTTTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A345CDT0

Antitoxin

Source ID Structure
AlphaFold DB A0A3E1ZZ82

References