Detailed information of TA system TA216920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1710974..1712539
• Replicon: chromosome
• Accession: NZ_CP083630
• Organism: Leclercia adecarboxylata strain FDAARGOS_1505

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A855EHA8
• Locus tag: LA332_RS08210
• Protein ID: WP_032618097.1
• Coordinates: 1711229..1712539 (+)
• Length: 437 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LA332_RS08205
• Protein ID: WP_032618098.1
• Coordinates: 1710974..1711225 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA332_RS08200 (LA332_08200)	1706374..1710174	-	3801	WP_032618099.1	glycosyltransferase	-
LA332_RS08205 (LA332_08205)	1710974..1711225	+	252	WP_032618098.1	helix-turn-helix domain-containing protein	Antitoxin
LA332_RS08210 (LA332_08210)	1711229..1712539	+	1311	WP_032618097.1	type II toxin-antitoxin system HipA family toxin	Toxin
LA332_RS08215 (LA332_08215)	1712675..1713736	+	1062	WP_032618096.1	YncE family protein	-
LA332_RS08220 (LA332_08220)	1713747..1715744	+	1998	WP_032618095.1	TonB-dependent receptor	-
LA332_RS08225 (LA332_08225)	1715877..1717058	+	1182	WP_032618094.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (151-399)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 437 a.a.
• Molecular weight: 48222.39 Da
• Isoelectric Point: 6.5044

>T216920 WP_032618097.1 NZ_CP083630:1711229-1712539 [Leclercia adecarboxylata]
MRAQRLTVAMNGDVVGTLHRDGSGAMSFQYAPEWLAEPGSRAISLSLPLQHGRIRGAQVFNFFSNLLPDSEAIIARMQAR
FQVETAHPFDLLASVGRDCVGAIQLYPPGQEIPPVTDIVAEPLDDDQIAALLDGYQMAPLGMTEEDADFRISLAGAQEKT
ALLWYQARWQRPQGSTPTSHIFKLPIGRIEQNNIDLSESCENEWLCLRIAKAFGFPVAQAEMATFGRKKVLIIERFDRRW
ASSGWLMRLPQEDFCQALGVSPALKYESHGGPGIADAMKLLLGSRLAGQDRELFFKAQILFWMLAAIDGHGKNFSLFIEP
DSAFRMAPLYDVISAFPLFDAGGIPVKKAKMAMALQGKNRQYHFAMIQPRHFISTAAFAGYSQEAARRLLTEMAERTDDV
IASVRAELPPDFPAQVSEAIFKGLASQAARIGRFVS

Nucleotide

• Length: 1311 bp

>T216920 NZ_CP113517:3771466-3771648 [Methylomonas rapida]
ATGAACAGCAAGCAACTGATTAAGCAACTGGAACAAGACGGCTGGTATCTGGCGCGGGTCAAAGGCTCACACCACCAATT
CAAGCACCCCACCAAACCCGGTTTGGTCACCGTCAAGCATCCAGACAGCGACATTCCAAAACCCACGTTGCACAGTATTT
ACCGGCAAGCCGGTTGGCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.56 Da
• Isoelectric Point: 10.1945

>AT216920 WP_032618098.1 NZ_CP083630:1710974-1711225 [Leclercia adecarboxylata]
MKVTSADALAQAVRDQRKVNKLTQTATAEQVGIKQTTVSEFENKPGSTKLETLFKILSALDLELHVVKRGGTLPDNNAWK
KEW

Nucleotide

• Length: 252 bp

>AT216920 NZ_CP113517:3771668-3772060 [Methylomonas rapida]
ATGAAATTTACCGTTGTTTTGCATACTGATGATGGTCATCATTACGGGGTCATCGTGCCTGATCTTCCCGGCTGTTTTTC
CGCCGGTGACGGCATCGATGAGGCGCTGTCATCCGTCATAGAAGCCATTGACCTGCATGTTGAAACATTGCTGGAAGACG
GCGGCGATATTCCGGCACGTTTGCCCATCGATGCGCACAAAGACAATCCTGATTTTGCCGGCGGTATTTGGGCGGTCGTT
GACGCGCCCATTGAAAAATACTTTGGCCCGGCGGAAAAAATCAACATTACCTTACCCAAGCTGTTACTGGCCAAGATCGA
TAGCTATGCTAAGGCGCATGGCGCTACCCGCTCAGGGTTTCTGGCCGATGCGGCGCGCTCTGCCATGCGGTAA

Structures

Toxin

• AlphaFold DB: A0A855EHA8