Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1633219..1633793 Replicon chromosome
Accession NZ_CP083630
Organism Leclercia adecarboxylata strain FDAARGOS_1505

Toxin (Protein)


Gene name mazF Uniprot ID A0A855EFR2
Locus tag LA332_RS07895 Protein ID WP_032618183.1
Coordinates 1633219..1633545 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A855EHH1
Locus tag LA332_RS07900 Protein ID WP_032618184.1
Coordinates 1633545..1633793 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA332_RS07875 (LA332_07875) 1628743..1629711 + 969 WP_032618179.1 ABC transporter substrate-binding protein -
LA332_RS07880 (LA332_07880) 1629750..1631405 + 1656 WP_098946240.1 iron ABC transporter permease -
LA332_RS07885 (LA332_07885) 1631602..1632447 + 846 WP_032618181.1 N-hydroxyarylamine O-acetyltransferase -
LA332_RS07890 (LA332_07890) 1632681..1633130 + 450 WP_032618182.1 hypothetical protein -
LA332_RS07895 (LA332_07895) 1633219..1633545 - 327 WP_032618183.1 endoribonuclease MazF Toxin
LA332_RS07900 (LA332_07900) 1633545..1633793 - 249 WP_032618184.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LA332_RS07905 (LA332_07905) 1633974..1634654 - 681 WP_032618185.1 respiratory nitrate reductase subunit gamma -
LA332_RS07910 (LA332_07910) 1634651..1635346 - 696 WP_032618186.1 nitrate reductase molybdenum cofactor assembly chaperone -
LA332_RS07915 (LA332_07915) 1635346..1636890 - 1545 WP_032618187.1 nitrate reductase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-107)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11865.61 Da        Isoelectric Point: 7.8831

>T216919 WP_032618183.1 NZ_CP083630:c1633545-1633219 [Leclercia adecarboxylata]
MDYIPEAGDIVWLDFDPQSGHEQAGHRPALVVSTATYNGRTGLMLCCPVTSQIKNYPFEVVISGARRNVALADQIKSLDW
RARHAVKKGTATAEELDAVRMRAKVLIG

Download         Length: 327 bp

>T216919 NZ_CP113517:3151027-3151281 [Methylomonas rapida]
ATGATTTTGGCTTGGGCGACAAATGCTTGGGACGATTACTTGTACTGGCAGGCCACCGATAAAAAGGTGCTGAAACGTAT
CAATACCTTGATCGAAGACATCAAACGCCAGCCATTCACCGGCATTGGCGATCCGGAGCTATTAAAGCACAACTGGTCCG
GATACTGGTCCAGGCGCATCGACCGTGAACATCGGTTGGTTTACAAGGCGGATGCCACCACGATTACCATCGTGCAATGT
CGCTATCACTACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9167.42 Da        Isoelectric Point: 4.4777

>AT216919 WP_032618184.1 NZ_CP083630:c1633793-1633545 [Leclercia adecarboxylata]
MPQVTVKKWGNSPSVRLPVAVMREAELHVDDVVNVNVDEEGRIILTPVRKDEPTLESLLAAITDDNLHHEVTFGEPQSKE
LL

Download         Length: 249 bp

>AT216919 NZ_CP113517:3150776-3151030 [Methylomonas rapida]
ATGGATACCATCAATTACACAACCTTGCGCAACAACCTCGCCAGCGTGCTTGATAAAGTCAACGACGACCATAACCCGGT
CTTGGTGACACGGCAAAATGGCAAACCGGCGGTGATCTTGTCGCTGGAAGATTACAAATCCTTCGAGGAAACCGCTTATT
TGATGGCCAGCCCGGTCAATGCGCAGCGTTTGAACCAAGCAATTGCGGAAGTGGAAGCCGGTAATACCGTAAACCATGAA
CTGATAGAACCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855EFR2

Antitoxin

Source ID Structure
AlphaFold DB A0A855EHH1

References