Detailed information of TA system TA216917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1077285..1078024
• Replicon: chromosome
• Accession: NZ_CP083630
• Organism: Leclercia adecarboxylata strain FDAARGOS_1505

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LA332_RS05210
• Protein ID: WP_058675171.1
• Coordinates: 1077539..1078024 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A085GLQ4
• Locus tag: LA332_RS05205
• Protein ID: WP_032611697.1
• Coordinates: 1077285..1077551 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA332_RS05185 (LA332_05185)	1072697..1072900	-	204	WP_032611702.1	hypothetical protein	-
LA332_RS05190 (LA332_05190)	1074037..1074785	-	749	Protein_1008	SDR family oxidoreductase	-
LA332_RS05195 (LA332_05195)	1074882..1075223	+	342	WP_032611701.1	helix-turn-helix domain-containing protein	-
LA332_RS05200 (LA332_05200)	1075694..1076932	-	1239	WP_032611699.1	IS110 family transposase	-
LA332_RS05205 (LA332_05205)	1077285..1077551	+	267	WP_032611697.1	DUF1778 domain-containing protein	Antitoxin
LA332_RS05210 (LA332_05210)	1077539..1078024	+	486	WP_058675171.1	GNAT family N-acetyltransferase	Toxin
LA332_RS05215 (LA332_05215)	1078443..1078736	+	294	Protein_1013	helix-turn-helix domain-containing protein	-
LA332_RS05220 (LA332_05220)	1078819..1079966	+	1148	WP_098946250.1	IS3 family transposase	-
LA332_RS05225 (LA332_05225)	1079982..1080593	-	612	WP_032610800.1	DNA (cytosine-5-)-methyltransferase	-
LA332_RS05230 (LA332_05230)	1080999..1081988	-	990	WP_058675112.1	tyrosine-type recombinase/integrase	-
LA332_RS05235 (LA332_05235)	1082130..1082609	+	480	Protein_1017	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17692.47 Da
• Isoelectric Point: 9.8719

>T216917 WP_058675171.1 NZ_CP083630:1077539-1078024 [Leclercia adecarboxylata]
VGHVTAPEPLSAFHQVAEFVSGETVLDDWLKQKGLKNQALGAARTFVVCKKDTKQIAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T216917 NZ_CP113517:c2316688-2316434 [Methylomonas rapida]
ATGAAACGCAAACACCAAAAAACCATCGAGGCGCTTTTCGCGCATCCTGTCGGCGCGAATATCCCATGGCGCGACATCGA
GTCGCTGTTTCTGGAGCTGGGCGCAACGGTCAGTGAACGCGAAGGTTCACGGGTTTGCGTGGTATTGTTTGGCCAGGTGC
GCGTTTTTCACCGCCCGCATCCATCGCCCAACACCGATAAAGGCGCGGTGGCCAGCGTGCGCGACTGGCTTAAATCTCAC
GGAGTACATCCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10164.64 Da
• Isoelectric Point: 4.3837

>AT216917 WP_032611697.1 NZ_CP083630:1077285-1077551 [Leclercia adecarboxylata]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPIIEKLL
ARKPQWDM

Nucleotide

• Length: 267 bp

>AT216917 NZ_CP113517:c2316437-2316102 [Methylomonas rapida]
ATGATTAATCAAATGATGATAGCCGGCGTTAAGGCCGTGATTACTTACGATTCCGACCTCGAGTTGTTTCGCGGTGAGTT
TGTGGGCTTGAGCGGCAGCGCGGACTTTTATGCCGCCGACGTGGCTGGATTGAAGCGCGAAGGTGAAATTTCTTTGCGCG
TGTATCTTGAAGCCTGCCAAGAATCCGGCATAGAACCGTTCAAGGCCTTTTCCGGAAAATTCAACGTGCGCATCCCCGCC
GATTTGCATGCCAGCATCGTCAGCGCGGCGGCGGCCCAAGGCAAAAGCATCAATCAATGGATTGGCGAGGCCTTGAGCCG
CGAGGTGAATGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A085GLQ4