Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 202476..204099 Replicon plasmid unnamed
Accession NZ_CP083621
Organism Enterobacter bugandensis strain FDAARGOS_1520

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LA316_RS23605 Protein ID WP_000088044.1
Coordinates 202476..203801 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LA316_RS23610 Protein ID WP_012477377.1
Coordinates 203806..204099 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA316_RS23575 (LA316_23575) 198462..199319 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
LA316_RS23580 (LA316_23580) 199335..199643 - 309 WP_001224686.1 hypothetical protein -
LA316_RS23585 (LA316_23585) 200192..200617 - 426 WP_000278471.1 hypothetical protein -
LA316_RS23590 (LA316_23590) 200870..201643 - 774 WP_001139513.1 HNH endonuclease signature motif containing protein -
LA316_RS23595 (LA316_23595) 201698..202108 - 411 WP_000985909.1 hypothetical protein -
LA316_RS23600 (LA316_23600) 202209..202415 - 207 WP_000134171.1 hypothetical protein -
LA316_RS23605 (LA316_23605) 202476..203801 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LA316_RS23610 (LA316_23610) 203806..204099 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LA316_RS23615 (LA316_23615) 204500..205888 - 1389 WP_000083579.1 hypothetical protein -
LA316_RS23620 (LA316_23620) 205893..206390 - 498 WP_000340139.1 membrane protein -
LA316_RS23625 (LA316_23625) 206593..207249 - 657 WP_000462754.1 trypsin-like serine protease -
LA316_RS23630 (LA316_23630) 207908..208339 - 432 WP_011152969.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / ant(3'')-Ia / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / qnrB1 / dfrA14 / tet(A) - 1..298988 298988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T216902 WP_000088044.1 NZ_CP083621:c203801-202476 [Enterobacter bugandensis]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T216902 NZ_CP113504:1084490-1084993 [Pectobacterium brasiliense]
ATGCATCTTGGTTTAACAGGGCTATATCAGCCAAAGTGGACGGCACTAATTCAGGAAGAGTGGCAGCGAAATTTGTTGCT
AAATAATGCAGACGTAACGATGGATAAGCTCAGGAACATTGAGCATTTGATGAATAAGGCATTACCTGATGCCAATGTGA
CTGGGTTCGAACAACTTATCCCTGCTCTGGTTTTACCTGATGCGGATGATCGACATGTATTGGCCGCTGCGATACGCGCT
AATGCAGAAGTTATCGTCACGGCAAATCTGAAAGATTTTCCAGCCACGTCACTTTCCGAATTTGGAGTGGAAGCCATGCA
CCCAGACGAATTCATTGCCGATTTATTTGATCTCAATTCTGCACTGGTGCTTAAAGCCGTTCGAACTTCGCGAACAGCCT
TCAGAAAGCCTCCATTTGATGTGGACGAGTATCTGGATTGTCTCCTGCGTCAGGGGTTACCTATGACGGTAAAAGAACTG
GATGCGTATCGAGCACTGATTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT216902 WP_012477377.1 NZ_CP083621:c204099-203806 [Enterobacter bugandensis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT216902 NZ_CP113504:1083957-1084418 [Pectobacterium brasiliense]
ATGAAAAATACTCCTCTAAATAGTTTGAGCCTTCCGGCTCCAAAAGAAATGGAGGCCGCTATCCGTGGACAAAGGCAGCT
TGCAACTTACCTGTCTACGAAGCTGGAAACACAGGAAATTTCGATTCAGGATGCGCACGACATTGCCCATCGGATCGAGT
TGCCTACCTCAGCACTCACGCTTTTGATGAGTATCCTTGGCGAACTGGCGGCGGGTAATGCTGTCCAGATTGTCCCTGTG
CAGGCTGAGTTAACGACACAAGAAGCGGCAAATATTCTGAATGTTTCACGGCCACATATGGTGAAGCTTTTGGAAGAAGG
CCATCTTCCTTTCCATAAAACAGGTCGTCATCGCCGCGTGCTTTTTGCTGATTTGATGCAATACAAAGAACAGCGTGAAA
ATGAAAGTATGCAAGCCATGCAGGAGCTGACAGACCGAAGTCAGGAATTCGGGTTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References