Detailed information of TA system TA216900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3576486..3577106
• Replicon: chromosome
• Accession: NZ_CP083620
• Organism: Enterobacter bugandensis strain FDAARGOS_1520

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: LA316_RS17075
• Protein ID: WP_008499287.1
• Coordinates: 3576888..3577106 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8E1RNX1
• Locus tag: LA316_RS17070
• Protein ID: WP_028012188.1
• Coordinates: 3576486..3576860 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA316_RS17060 (LA316_17060)	3571614..3572807	+	1194	WP_028012190.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
LA316_RS17065 (LA316_17065)	3572830..3575976	+	3147	WP_047367620.1	multidrug efflux RND transporter permease subunit AcrB	-
LA316_RS17070 (LA316_17070)	3576486..3576860	+	375	WP_028012188.1	Hha toxicity modulator TomB	Antitoxin
LA316_RS17075 (LA316_17075)	3576888..3577106	+	219	WP_008499287.1	HHA domain-containing protein	Toxin
LA316_RS17080 (LA316_17080)	3577316..3577867	+	552	WP_028012187.1	maltose O-acetyltransferase	-
LA316_RS17085 (LA316_17085)	3577984..3578451	+	468	WP_028012186.1	YlaC family protein	-
LA316_RS17090 (LA316_17090)	3578423..3579883	-	1461	WP_088205222.1	PLP-dependent aminotransferase family protein	-
LA316_RS17095 (LA316_17095)	3579985..3580695	+	711	WP_088205223.1	GNAT family protein	-
LA316_RS17100 (LA316_17100)	3580692..3580832	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
LA316_RS17105 (LA316_17105)	3580835..3581095	-	261	WP_006176933.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T216900 WP_008499287.1 NZ_CP083620:3576888-3577106 [Enterobacter bugandensis]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T216900 NZ_CP113504:1018168-1018572 [Pectobacterium brasiliense]
ATGAGCATCAGGATTTTCAAGTCCACGTTGCTTTGTCAACAATTGAGCCAGCAAGAGCTGGACGATTTAGTTGCGGACTT
CCTGTCTTATAAACAGGATGGCGTGTTACCAGATACTTTTGGCCGTGATGCGCTCTATGATGATGATCGCACCTACCCAT
TGGTAAGAGAAGAACAAGTAGCCCACATCCATCTTGCTGATGCGAATGCTCCTTTCCCCAAGTTCTTACGCCAGTTCAAA
AGAACCAGCGATAAAGCCCATTTGGTTTACTGCCAAAGTGCGATGGATCCTGATATCTATCTGCTGATAATCATTTTAAA
GCCTGAAGCGCACAAGATGGCACGAGACAATAACAATATGCACAAGGTTGGGATGATGGCAGAAGCGTTCAGAATGAAAT
ATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.30 Da
• Isoelectric Point: 4.8886

>AT216900 WP_028012188.1 NZ_CP083620:3576486-3576860 [Enterobacter bugandensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT216900 NZ_CP113504:1017878-1018171 [Pectobacterium brasiliense]
ATGGAGCGCATTCTTGCTGAGAAATCGATTAACATCACTGAGTTAAGAAAAAACCCAGCCAAGTATTTTATTGATGAACC
CGTTGCCGTGCTTTCTAACAATCGGCCTGCCGGTTACATGGTCAGTGCCAAGGTCTTTGAAGCTCTGATTGACCTATTGG
AAGATAAGCAGGGTAGCGTTCATACCGCAGCACGTTTCAGGCCAACCGCTGAACGTTTAAGTAATATTGCGGATAATGGG
GTGACGCTGCTGAAAAATGCTTCTGATAAGGATCTGAACGAGTTCACGGAATGA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38