Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/DUF1778(antitoxin) |
| Location | 2985047..2985844 | Replicon | chromosome |
| Accession | NZ_CP083620 | ||
| Organism | Enterobacter bugandensis strain FDAARGOS_1520 | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | - |
| Locus tag | LA316_RS14270 | Protein ID | WP_172616463.1 |
| Coordinates | 2985047..2985547 (-) | Length | 167 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | W7PG43 |
| Locus tag | LA316_RS14275 | Protein ID | WP_008499829.1 |
| Coordinates | 2985575..2985844 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LA316_RS14240 (LA316_14240) | 2980645..2981334 | + | 690 | WP_028012657.1 | pyrimidine utilization protein B | - |
| LA316_RS14245 (LA316_14245) | 2981346..2981732 | + | 387 | WP_088204983.1 | pyrimidine utilization protein C | - |
| LA316_RS14250 (LA316_14250) | 2981740..2982540 | + | 801 | WP_028012656.1 | pyrimidine utilization protein D | - |
| LA316_RS14255 (LA316_14255) | 2982550..2983140 | + | 591 | WP_041910551.1 | malonic semialdehyde reductase | - |
| LA316_RS14260 (LA316_14260) | 2983150..2983644 | + | 495 | WP_028012654.1 | pyrimidine utilization flavin reductase protein F | - |
| LA316_RS14265 (LA316_14265) | 2983666..2984988 | + | 1323 | WP_048959639.1 | pyrimidine utilization transport protein G | - |
| LA316_RS14270 (LA316_14270) | 2985047..2985547 | - | 501 | WP_172616463.1 | GNAT family N-acetyltransferase | Toxin |
| LA316_RS14275 (LA316_14275) | 2985575..2985844 | - | 270 | WP_008499829.1 | DUF1778 domain-containing protein | Antitoxin |
| LA316_RS14280 (LA316_14280) | 2985906..2986823 | - | 918 | WP_088204981.1 | DMT family transporter | - |
| LA316_RS14285 (LA316_14285) | 2986940..2987110 | - | 171 | WP_001273664.1 | general stress protein | - |
| LA316_RS14290 (LA316_14290) | 2987501..2988097 | + | 597 | WP_041910554.1 | NAD(P)H:quinone oxidoreductase | - |
| LA316_RS14295 (LA316_14295) | 2988116..2988343 | + | 228 | WP_028012649.1 | YccJ family protein | - |
| LA316_RS14300 (LA316_14300) | 2988377..2989618 | - | 1242 | WP_088204980.1 | bifunctional glucose-1-phosphatase/inositol phosphatase | - |
| LA316_RS14305 (LA316_14305) | 2989771..2990214 | + | 444 | WP_028012647.1 | helix-turn-helix domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 167 a.a. Molecular weight: 18873.63 Da Isoelectric Point: 8.4785
>T216898 WP_172616463.1 NZ_CP083620:c2985547-2985047 [Enterobacter bugandensis]
MFSEGTDYDFGDFDCGEPSLNAFLREHLVRQHNGRILRAYLLKERDHPRVLGYYTLSGSCFERAMLPSKTQQRRIPYLNV
PSVTLGRLAIHKELQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALSEQAKRFYLKLGFIPLAGENSHSLFFPTKAIE
RLFEQA
MFSEGTDYDFGDFDCGEPSLNAFLREHLVRQHNGRILRAYLLKERDHPRVLGYYTLSGSCFERAMLPSKTQQRRIPYLNV
PSVTLGRLAIHKELQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALSEQAKRFYLKLGFIPLAGENSHSLFFPTKAIE
RLFEQA
Download Length: 501 bp
>T216898 NZ_CP113504:763902-764222 [Pectobacterium brasiliense]
ATGCAAACATCACCTGCAATCCCGCCACGGGAGGATAACCCCTGTCCGTCACCTATAGCCGTCTGGCAGCAACTGCTTAC
CTACCTGTTAGAAAAGCACTACGGCCTGACGCTGAACGACACACCGTTCTGTGAGGAAAACGTCATTCAGGCGCATATCG
ATGCTGGCGTCACGTTGGTCAATGCCGTGAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTTACCGCCGTTGATGTTCTCCGTGCCAGACGTGCTACCGGCTTGCTCAAGGCATA
A
ATGCAAACATCACCTGCAATCCCGCCACGGGAGGATAACCCCTGTCCGTCACCTATAGCCGTCTGGCAGCAACTGCTTAC
CTACCTGTTAGAAAAGCACTACGGCCTGACGCTGAACGACACACCGTTCTGTGAGGAAAACGTCATTCAGGCGCATATCG
ATGCTGGCGTCACGTTGGTCAATGCCGTGAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTTACCGCCGTTGATGTTCTCCGTGCCAGACGTGCTACCGGCTTGCTCAAGGCATA
A
Antitoxin
Download Length: 90 a.a. Molecular weight: 10262.72 Da Isoelectric Point: 5.8836
>AT216898 WP_008499829.1 NZ_CP083620:c2985844-2985575 [Enterobacter bugandensis]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME
Download Length: 270 bp
>AT216898 NZ_CP113504:763497-763832 [Pectobacterium brasiliense]
ATGTCATCAATAGAGGCCCCGGAATGGGGATTAAAGTGTACTGTCACGCCGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCACTATCTGGCAGACCGGGCCAGCATTGTCGGTACGTTTAGCAAAACAGAAGCCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTGATTAAGCTGTTGGAGCAGAAGCTACGCATGGGGGAACTGAATCCCCGTCAGCAGGGTTGCATCACG
TTGTATTGCAACGCATGGACCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTATATATTGCTATTTATCCCTCTTC
TTCGACAGCAGAATAA
ATGTCATCAATAGAGGCCCCGGAATGGGGATTAAAGTGTACTGTCACGCCGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCACTATCTGGCAGACCGGGCCAGCATTGTCGGTACGTTTAGCAAAACAGAAGCCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTGATTAAGCTGTTGGAGCAGAAGCTACGCATGGGGGAACTGAATCCCCGTCAGCAGGGTTGCATCACG
TTGTATTGCAACGCATGGACCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTATATATTGCTATTTATCCCTCTTC
TTCGACAGCAGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|