Detailed information of TA system TA021688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 71463..72099
• Replicon: chromosome
• Accession: NC_008344
• Organism: Nitrosomonas eutropha C91

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q0AJW2
• Locus tag: NEUT_RS00375
• Protein ID: WP_011633204.1
• Coordinates: 71683..72099 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q0AJW3
• Locus tag: NEUT_RS00370
• Protein ID: WP_011633203.1
• Coordinates: 71463..71696 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NEUT_RS00360	68874..69281	+	408	WP_049753072.1	Cu(I)-responsive transcriptional regulator	-
NEUT_RS00365	69328..71193	-	1866	WP_011633202.1	TraI domain-containing protein	-
NEUT_RS00370	71463..71696	+	234	WP_011633203.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
NEUT_RS00375	71683..72099	+	417	WP_011633204.1	PIN domain-containing protein	Toxin
NEUT_RS00380	72119..72487	+	369	WP_041353624.1	DUF3742 family protein	-
NEUT_RS00385	72484..73989	-	1506	WP_011633206.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
NEUT_RS00390	74005..74349	-	345	WP_011633207.1	hypothetical protein	-
NEUT_RS00395	74346..75743	-	1398	WP_011633208.1	integrating conjugative element protein	-
NEUT_RS00400	75762..76694	-	933	WP_011633209.1	TIGR03756 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	10240..83807	73567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15521.97 Da
• Isoelectric Point: 6.4724

>T21688 WP_011633204.1 NC_008344:71683-72099 [Nitrosomonas eutropha C91]
MRVTKFKHFMDSNVVLYLLSEDSTKADKAESLLKAGPVISVQVLNEVTNVCVRKLKMSWDEIAQFLELIRSFCKVVPLTE
TIHDQARLIAERHKLSFYDSCIVAAAVAAGCQTLYSEDMNHGQILEDSLSLRNPFSTV

Nucleotide

• Length: 417 bp

>T21688 NC_008344:71683-72099 [Nitrosomonas eutropha C91]
ATGCGCGTGACTAAGTTCAAGCATTTTATGGACAGCAATGTGGTGTTGTACCTGCTGTCGGAGGACTCGACCAAAGCGGA
CAAGGCCGAAAGCCTGCTGAAAGCCGGGCCAGTCATCAGCGTGCAAGTGCTCAACGAAGTGACGAATGTCTGCGTACGCA
AGCTCAAAATGAGCTGGGACGAGATCGCGCAGTTCCTGGAACTGATACGGAGCTTTTGCAAGGTCGTCCCACTCACGGAA
ACCATCCATGATCAGGCCCGATTGATCGCTGAGCGACATAAGCTGTCCTTCTACGACTCCTGCATCGTCGCAGCAGCTGT
GGCAGCGGGTTGCCAGACGCTCTATAGTGAGGACATGAACCACGGGCAGATACTGGAAGATAGCTTGAGTCTCAGGAATC
CATTTTCCACCGTATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8563.83 Da
• Isoelectric Point: 10.1756

>AT21688 WP_011633203.1 NC_008344:71463-71696 [Nitrosomonas eutropha C91]
MQVAKWGNSLAVRLPASLVEALELQEGDDIEIVVDGPRAFAVRKKPGADALLKRLRTFRGKLPADFRFSRDEANARD

Nucleotide

• Length: 234 bp

>AT21688 NC_008344:71463-71696 [Nitrosomonas eutropha C91]
ATGCAAGTCGCCAAATGGGGCAACAGCTTGGCCGTGCGCCTTCCGGCCAGCCTGGTAGAAGCGCTGGAGTTGCAAGAAGG
TGACGATATTGAGATCGTCGTGGACGGCCCGCGCGCGTTCGCGGTTCGCAAAAAGCCAGGCGCCGATGCCTTGTTGAAGC
GCCTGCGCACCTTCCGGGGCAAACTGCCGGCGGATTTCCGCTTCAGCCGGGACGAGGCCAATGCGCGTGACTAA

Structures

Toxin

• AlphaFold DB: Q0AJW2

Antitoxin

• AlphaFold DB: Q0AJW3