Detailed information of TA system TA216876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 3343656..3345439
• Replicon: chromosome
• Accession: NZ_CP083613
• Organism: Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LA353_RS16080
• Protein ID: WP_047053382.1
• Coordinates: 3343656..3345005 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LA353_RS16085
• Protein ID: WP_047053381.1
• Coordinates: 3345002..3345439 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA353_RS16055 (LA353_16050)	3339479..3340414	-	936	WP_069597669.1	tRNA dihydrouridine(16) synthase DusC	-
LA353_RS16060 (LA353_16055)	3340583..3340978	+	396	WP_023295055.1	CidA/LrgA family protein	-
LA353_RS16065 (LA353_16060)	3340975..3341670	+	696	WP_015572322.1	CidB/LrgB family autolysis modulator	-
LA353_RS16070 (LA353_16065)	3341799..3342683	+	885	WP_047053384.1	cytidine deaminase	-
LA353_RS16075 (LA353_16070)	3342811..3343521	+	711	WP_003859314.1	outer membrane permeability protein SanA	-
LA353_RS16080 (LA353_16075)	3343656..3345005	+	1350	WP_047053382.1	HipA domain-containing protein	Toxin
LA353_RS16085 (LA353_16080)	3345002..3345439	+	438	WP_047053381.1	helix-turn-helix transcriptional regulator	Antitoxin
LA353_RS16090 (LA353_16085)	3345478..3346488	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
LA353_RS16095 (LA353_16090)	3346504..3348024	-	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
LA353_RS16100 (LA353_16095)	3348104..3349102	-	999	WP_017383382.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
LA353_RS16105 (LA353_16100)	3349398..3350420	-	1023	WP_047053378.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-129)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51132.76 Da
• Isoelectric Point: 4.6556

>T216876 WP_047053382.1 NZ_CP083613:3343656-3345005 [Enterobacter hormaechei subsp. oharae]
METLTVQAYLNETWTDIALIKYPGSEKNDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLTCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFHYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T216876 NZ_CP113503:2272065-2272167 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16161.57 Da
• Isoelectric Point: 10.5154

>AT216876 WP_047053381.1 NZ_CP083613:3345002-3345439 [Enterobacter hormaechei subsp. oharae]
MKTSNEAESELLQTLAQVRSVNKKRHHDEAEDPDKPSVVKRQRVSQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADIINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT216876 NZ_CP113503:c2272205-2271937 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT