Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2020280..2021077 Replicon chromosome
Accession NZ_CP083613
Organism Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag LA353_RS09575 Protein ID WP_032670168.1
Coordinates 2020556..2021077 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag LA353_RS09570 Protein ID WP_001303459.1
Coordinates 2020280..2020549 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA353_RS09535 (LA353_09530) 2015820..2016179 + 360 WP_040117469.1 helix-turn-helix domain-containing protein -
LA353_RS09540 (LA353_09535) 2016251..2016352 - 102 Protein_1839 transcriptional regulator -
LA353_RS09545 (LA353_09540) 2016505..2017746 + 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
LA353_RS09550 (LA353_09545) 2017779..2018006 - 228 WP_006809324.1 YccJ family protein -
LA353_RS09555 (LA353_09550) 2018025..2018621 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
LA353_RS09560 (LA353_09555) 2019013..2019183 + 171 WP_001273664.1 general stress protein -
LA353_RS09565 (LA353_09560) 2019300..2020205 + 906 WP_047051684.1 DMT family transporter -
LA353_RS09570 (LA353_09565) 2020280..2020549 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
LA353_RS09575 (LA353_09570) 2020556..2021077 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
LA353_RS09580 (LA353_09575) 2021136..2022458 - 1323 WP_048982825.1 pyrimidine utilization transport protein G -
LA353_RS09585 (LA353_09580) 2022480..2022971 - 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
LA353_RS09590 (LA353_09585) 2022984..2023574 - 591 WP_047052687.1 malonic semialdehyde reductase -
LA353_RS09595 (LA353_09590) 2023584..2024384 - 801 WP_047052686.1 pyrimidine utilization protein D -
LA353_RS09600 (LA353_09595) 2024392..2024778 - 387 WP_032670161.1 pyrimidine utilization protein C -
LA353_RS09605 (LA353_09600) 2024790..2025479 - 690 WP_047052685.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T216865 WP_032670168.1 NZ_CP083613:2020556-2021077 [Enterobacter hormaechei subsp. oharae]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T216865 NZ_CP113499:c791448-791152 [Mycoplasma feriruminatoris]
ATGACTAAATATAAAATTGTTTGAACAACAAAATTTAAAAAGGATTTAAAGCTAGCTAAAAAACAGAATAAAGATTTAAA
TAAACTAGACTATGTTATTAATATATTAATTCAAGGCAAAGAGTTAGACGCAAAATATCAGGATCATTCACTTACTGGCA
TTTATAAAGAATATAGAGAGTGTCATATTGACCCTGACTGACTTTTAATATATAAAAAAGATGATGATAAAATTGTTATA
ATAATGTTAAGATTAGGTTCACATTCTAATTTATTTGAAAAACAAAAAAACTACTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT216865 WP_001303459.1 NZ_CP083613:2020280-2020549 [Enterobacter hormaechei subsp. oharae]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT216865 NZ_CP113499:c791713-791441 [Mycoplasma feriruminatoris]
ATGAAAACAACAAATTTAAACGTAAGAATTGATAAAGAAGTCAAACATGATGCAGAAGCTATATTTAATGAATTGGGTTT
AAGCACATCAGCAGCTATAAATATGTTTTTTAAGGCTACAATTAGAAATAACAGTCTTCCTTTTAGTACTAAACTGGATA
CTCCTAATAAAGAAACTTTGTTAGCTATTAAAGAAGCAGAAGAACTATTAAAAGATCCAAATACAAAGTATTATTCAAGC
TTTAAAGAACTTTTAGAGACATTAGATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References