Detailed information of TA system TA216857

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 366007..366674
• Replicon: chromosome
• Accession: NZ_CP083613
• Organism: Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: LA353_RS01645
• Protein ID: WP_059443702.1
• Coordinates: 366354..366674 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LA353_RS01640
• Protein ID: WP_059443453.1
• Coordinates: 366007..366333 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA353_RS01610 (LA353_01610)	361206..362585	-	1380	WP_015570106.1	carbohydrate porin	-
LA353_RS01615 (LA353_01615)	362722..363024	-	303	WP_014168006.1	PTS lactose/cellobiose transporter subunit IIA	-
LA353_RS01620 (LA353_01620)	363030..363230	-	201	Protein_306	family 1 glycosylhydrolase	-
LA353_RS01625 (LA353_01625)	363303..364423	+	1121	WP_085950818.1	IS3-like element ISSen4 family transposase	-
LA353_RS01630 (LA353_01630)	364634..365455	+	822	WP_015703839.1	DUF932 domain-containing protein	-
LA353_RS01635 (LA353_01635)	365524..365994	+	471	WP_015703840.1	DNA repair protein RadC	-
LA353_RS01640 (LA353_01640)	366007..366333	+	327	WP_059443453.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LA353_RS01645 (LA353_01645)	366354..366674	+	321	WP_059443702.1	TA system toxin CbtA family protein	Toxin
LA353_RS01650 (LA353_01650)	366969..368525	+	1557	WP_047050722.1	hypothetical protein	-
LA353_RS01655 (LA353_01655)	368565..368741	-	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
LA353_RS01660 (LA353_01660)	368878..369219	+	342	WP_006812446.1	SymE family type I addiction module toxin	-
LA353_RS01665 (LA353_01665)	369322..369692	+	371	Protein_315	hydroxyacid dehydrogenase	-
LA353_RS01670 (LA353_01670)	369847..370197	+	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LA353_RS01675 (LA353_01675)	370194..370493	+	300	WP_045335986.1	XRE family transcriptional regulator	-
LA353_RS01680 (LA353_01680)	370558..371439	-	882	WP_047050719.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	363303..384860	21557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-99)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12048.92 Da
• Isoelectric Point: 6.2120

>T216857 WP_059443702.1 NZ_CP083613:366354-366674 [Enterobacter hormaechei subsp. oharae]
MNTQPATTPQAAKPCLSPLAVWKMLLTILLDQHYGLTLNDTPFSDETVIQKHIEAGITLADAINFLVERYELVRIDQKGF
SVQDQEPWLTSMDVHRARFKLNVARL

Nucleotide

• Length: 321 bp

>T216857 NZ_CP113493:c4718181-4718008 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12277.99 Da
• Isoelectric Point: 6.3620

>AT216857 WP_059443453.1 NZ_CP083613:366007-366333 [Enterobacter hormaechei subsp. oharae]
MSLIPAHEWGLQREIVPRFGARLVQEGNRLHYLADRASLMGNFSDTECFKLNDAFPHFISQMESMLTTGELIPCHHHCVT
LYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 327 bp

>AT216857 NZ_CP113493:c4718505-4718209 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG