Detailed information of TA system TA216856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 227931..228547
• Replicon: chromosome
• Accession: NZ_CP083613
• Organism: Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LA353_RS00975
• Protein ID: WP_080480776.1
• Coordinates: 227931..228302 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: LA353_RS00980
• Protein ID: WP_015569912.1
• Coordinates: 228305..228547 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA353_RS00960 (LA353_00960)	225431..226333	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
LA353_RS00965 (LA353_00965)	226330..226965	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
LA353_RS00970 (LA353_00970)	226962..227891	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
LA353_RS00975 (LA353_00975)	227931..228302	-	372	WP_080480776.1	type II toxin-antitoxin system VapC family toxin	Toxin
LA353_RS00980 (LA353_00980)	228305..228547	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
LA353_RS00985 (LA353_00985)	228746..229666	+	921	WP_047051644.1	alpha/beta hydrolase	-
LA353_RS00990 (LA353_00990)	229675..230616	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
LA353_RS00995 (LA353_00995)	230661..231098	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
LA353_RS01000 (LA353_01000)	231095..231976	-	882	WP_003861949.1	virulence factor BrkB family protein	-
LA353_RS01005 (LA353_01005)	231970..232569	-	600	WP_032649898.1	glucose-1-phosphatase	-
LA353_RS01010 (LA353_01010)	232688..233488	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13792.96 Da
• Isoelectric Point: 6.7561

>T216856 WP_080480776.1 NZ_CP083613:c228302-227931 [Enterobacter hormaechei subsp. oharae]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQRRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T216856 NZ_CP113493:4706681-4707058 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT216856 WP_015569912.1 NZ_CP083613:c228547-228305 [Enterobacter hormaechei subsp. oharae]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT216856 NZ_CP113493:4706223-4706591 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3