Detailed information of TA system TA216853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 109455..110365
• Replicon: plasmid pVir-fusion
• Accession: NZ_CP083611
• Organism: Escherichia coli strain EC2648-Vir1

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LAJ48_RS22885
• Protein ID: WP_004026354.1
• Coordinates: 109455..109925 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LAJ48_RS22890
• Protein ID: WP_004026357.1
• Coordinates: 109922..110365 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ48_RS22860 (LAJ48_22870)	104867..105265	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
LAJ48_RS22865 (LAJ48_22875)	105602..106096	-	495	WP_004212794.1	thermonuclease family protein	-
LAJ48_RS22870 (LAJ48_22880)	106274..106540	+	267	WP_223175074.1	DUF1173 family protein	-
LAJ48_RS22875 (LAJ48_22885)	106573..107223	+	651	WP_068893702.1	DUF1173 family protein	-
LAJ48_RS22885 (LAJ48_22895)	109455..109925	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LAJ48_RS22890 (LAJ48_22900)	109922..110365	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LAJ48_RS22895 (LAJ48_22905)	110466..110969	-	504	Protein_113	DUF4113 domain-containing protein	-
LAJ48_RS22900 (LAJ48_22910)	111175..112155	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
LAJ48_RS22905 (LAJ48_22915)	112221..113528	+	1308	Protein_115	FepA family TonB-dependent siderophore receptor	-
LAJ48_RS22910 (LAJ48_22920)	113590..113811	+	222	WP_004213615.1	hypothetical protein	-
LAJ48_RS22915 (LAJ48_22925)	113954..114856	-	903	WP_004213613.1	DMT family inner membrane transporter PEG344	-
LAJ48_RS22920 (LAJ48_22930)	114929..115330	-	402	WP_214588034.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..255611	255611
	-	inside	IScluster/Tn	-	-	103416..112155	8739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T216853 WP_004026354.1 NZ_CP083611:c109925-109455 [Escherichia coli]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T216853 NZ_CP113493:c4359608-4359273 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT216853 WP_004026357.1 NZ_CP083611:c110365-109922 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT216853 NZ_CP113493:c4359856-4359608 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9