Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2326160..2326814 Replicon chromosome
Accession NZ_CP083610
Organism Escherichia coli strain EC2648-Vir1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LAJ48_RS11245 Protein ID WP_000244777.1
Coordinates 2326407..2326814 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LAJ48_RS11240 Protein ID WP_000354046.1
Coordinates 2326160..2326426 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ48_RS11215 (2321329) 2321329..2322072 + 744 WP_000951964.1 SDR family oxidoreductase -
LAJ48_RS11220 (2322129) 2322129..2323562 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LAJ48_RS11225 (2323607) 2323607..2323918 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LAJ48_RS11230 (2324082) 2324082..2324741 + 660 WP_072647361.1 hemolysin III family protein -
LAJ48_RS11235 (2324937) 2324937..2325917 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LAJ48_RS11240 (2326160) 2326160..2326426 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LAJ48_RS11245 (2326407) 2326407..2326814 + 408 WP_000244777.1 protein YgfX Toxin
LAJ48_RS11250 (2326854) 2326854..2327375 - 522 WP_001055874.1 flavodoxin FldB -
LAJ48_RS11255 (2327487) 2327487..2328383 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LAJ48_RS11260 (2328408) 2328408..2329118 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LAJ48_RS11265 (2329124) 2329124..2330857 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T216835 WP_000244777.1 NZ_CP083610:2326407-2326814 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216835 NZ_CP113493:217414-217521 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216835 WP_000354046.1 NZ_CP083610:2326160-2326426 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216835 NZ_CP113493:c217365-217302 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References