Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1010887..1011384 Replicon chromosome
Accession NZ_CP083610
Organism Escherichia coli strain EC2648-Vir1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LAJ48_RS04985 Protein ID WP_001173343.1
Coordinates 1010887..1011060 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LAJ48_RS04990 Protein ID WP_000398619.1
Coordinates 1011088..1011384 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ48_RS04975 (1007557) 1007557..1009014 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LAJ48_RS04980 (1009251) 1009251..1010768 + 1518 WP_001295074.1 lysine--tRNA ligase -
LAJ48_RS04985 (1010887) 1010887..1011060 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LAJ48_RS04990 (1011088) 1011088..1011384 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LAJ48_RS04995 (1011611) 1011611..1011883 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LAJ48_RS05000 (1011895) 1011895..1012125 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LAJ48_RS05005 (1012306) 1012306..1013937 + 1632 WP_001216477.1 sensor histidine kinase -
LAJ48_RS05010 (1013934) 1013934..1014653 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T216829 WP_001173343.1 NZ_CP083610:c1011060-1010887 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T216829 NZ_CP113487:c47893-47744 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT216829 WP_000398619.1 NZ_CP083610:c1011384-1011088 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT216829 NZ_CP113487:47937-47998 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References