Detailed information of TA system TA216822

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 402497..403176
• Replicon: chromosome
• Accession: NZ_CP083610
• Organism: Escherichia coli strain EC2648-Vir1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: LAJ48_RS01965
• Protein ID: WP_000854672.1
• Coordinates: 402497..402838 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: LAJ48_RS01970
• Protein ID: WP_000070395.1
• Coordinates: 402859..403176 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ48_RS01940 (398083)	398083..398175	+	93	Protein_383	sigma factor-binding protein Crl	-
LAJ48_RS01945 (398214)	398214..399269	-	1056	WP_000749863.1	phosphoporin PhoE	-
LAJ48_RS01950 (399557)	399557..400660	+	1104	WP_001285288.1	glutamate 5-kinase	-
LAJ48_RS01955 (400672)	400672..401925	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
LAJ48_RS01965 (402497)	402497..402838	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
LAJ48_RS01970 (402859)	402859..403176	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LAJ48_RS01975 (403195)	403195..403416	-	222	WP_000691994.1	DUF987 domain-containing protein	-
LAJ48_RS01980 (403425)	403425..403901	-	477	WP_000811693.1	RadC family protein	-
LAJ48_RS01985 (403917)	403917..404375	-	459	WP_000211838.1	antirestriction protein	-
LAJ48_RS01990 (404473)	404473..404712	-	240	WP_000194654.1	DUF905 family protein	-
LAJ48_RS01995 (404789)	404789..405256	-	468	WP_001547765.1	protein YkfB	-
LAJ48_RS02000 (405279)	405279..405722	-	444	WP_000824223.1	lipoprotein YafY	-
LAJ48_RS02005 (405722)	405722..405949	-	228	WP_001548158.1	protein YpjK	-
LAJ48_RS02010 (405945)	405945..406136	-	192	Protein_396	DeoR family transcriptional regulator	-
LAJ48_RS02015 (406353)	406353..407174	-	822	WP_000197389.1	DUF932 domain-containing protein	-
LAJ48_RS02020 (407266)	407266..408129	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	397377..436190	38813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T216822 WP_000854672.1 NZ_CP083610:c402838-402497 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T216822 NZ_CP113486:3931976-3932896 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTACA
AGAGCTTTGCGGATCGCGATACTGATTTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCTGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATCGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCGCCACGTTGTACGCGC
AGGAATTTTCAGGGATGACGCAACAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTTCTGGCGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT216822 WP_000070395.1 NZ_CP083610:c403176-402859 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT216822 NZ_CP113486:3931207-3931989 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684