Detailed information of TA system TA216805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 234910..236018
• Replicon: chromosome
• Accession: NZ_CP083594
• Organism: Macrococcus armenti strain 19Msa0295

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A2I4SCC6
• Locus tag: LAU38_RS01330
• Protein ID: WP_062810426.1
• Coordinates: 235149..236018 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: B9DSK1
• Locus tag: LAU38_RS01325
• Protein ID: WP_002303393.1
• Coordinates: 234910..235134 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAU38_RS01310 (LAU38_01310)	232334..232786	+	453	WP_223804552.1	DNA recombinase	-
LAU38_RS01315 (LAU38_01315)	232787..233203	+	417	WP_000323438.1	recombinase	-
LAU38_RS01320 (LAU38_01320)	233223..234767	+	1545	WP_224778340.1	recombinase family protein	-
LAU38_RS01325 (LAU38_01325)	234910..235134	+	225	WP_002303393.1	helix-turn-helix transcriptional regulator	Antitoxin
LAU38_RS01330 (LAU38_01330)	235149..236018	+	870	WP_062810426.1	nucleotidyltransferase domain-containing protein	Toxin
LAU38_RS01335 (LAU38_01335)	235999..236733	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LAU38_RS01340 (LAU38_01340)	236766..237629	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LAU38_RS01345 (LAU38_01345)	237673..238200	+	528	WP_224199246.1	phosphoribosyltransferase family protein	-
LAU38_RS01350 (LAU38_01350)	238428..239747	+	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	ant(6)-Ia	-	236766..239747	2981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32793.31 Da
• Isoelectric Point: 4.6575

>T216805 WP_062810426.1 NZ_CP083594:235149-236018 [Macrococcus armenti]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T216805 NZ_CP113486:c1541953-1541642 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8351.65 Da
• Isoelectric Point: 4.5357

>AT216805 WP_002303393.1 NZ_CP083594:234910-235134 [Macrococcus armenti]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Nucleotide

• Length: 225 bp

>AT216805 NZ_CP113486:c1541645-1541226 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A2I4SCC6

Antitoxin

• AlphaFold DB: A0A1M5SHM7