Detailed information of TA system TA216802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 237673..238781
• Replicon: chromosome
• Accession: NZ_CP083592
• Organism: Macrococcus armenti strain 19Msa0966

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A2H4HI44
• Locus tag: LAU41_RS01335
• Protein ID: WP_029170889.1
• Coordinates: 237912..238781 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LAU41_RS01330
• Protein ID: WP_000205227.1
• Coordinates: 237673..237897 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAU41_RS01315 (LAU41_01315)	235088..235549	+	462	WP_224199244.1	DNA recombinase	-
LAU41_RS01320 (LAU41_01320)	235550..235966	+	417	WP_000323438.1	recombinase	-
LAU41_RS01325 (LAU41_01325)	235986..237530	+	1545	Protein_261	recombinase family protein	-
LAU41_RS01330 (LAU41_01330)	237673..237897	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LAU41_RS01335 (LAU41_01335)	237912..238781	+	870	WP_029170889.1	nucleotidyltransferase domain-containing protein	Toxin
LAU41_RS01340 (LAU41_01340)	238762..239496	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LAU41_RS01345 (LAU41_01345)	239529..240392	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LAU41_RS01350 (LAU41_01350)	240436..240963	+	528	WP_224199246.1	phosphoribosyltransferase family protein	-
LAU41_RS01355 (LAU41_01355)	241191..242510	+	1320	WP_224199231.1	IS1380-like element ISSsu5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	mecD / ant(6)-Ia	-	228941..244352	15411
	-	flank	IS/Tn	ant(6)-Ia	-	239529..242510	2981

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32820.25 Da
• Isoelectric Point: 4.9564

>T216802 WP_029170889.1 NZ_CP083592:237912-238781 [Macrococcus armenti]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T216802 NZ_CP113486:720487-720861 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCTGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT216802 WP_000205227.1 NZ_CP083592:237673-237897 [Macrococcus armenti]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT216802 NZ_CP113486:720030-720398 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A2H4HI44