Detailed information of TA system TA216800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1597792..1599527
• Replicon: chromosome
• Accession: NZ_CP083589
• Organism: Herbaspirillum frisingense strain AU14559

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LAZ82_RS07100
• Protein ID: WP_234295920.1
• Coordinates: 1597792..1598679 (+)
• Length: 296 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1V2VDX6
• Locus tag: LAZ82_RS07105
• Protein ID: WP_006462350.1
• Coordinates: 1598694..1599527 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAZ82_RS07075 (LAZ82_07075)	1593664..1594449	+	786	WP_006462344.1	ABC transporter ATP-binding protein	-
LAZ82_RS07080 (LAZ82_07080)	1594505..1595500	+	996	WP_006462345.1	ABC transporter substrate-binding protein	-
LAZ82_RS07085 (LAZ82_07085)	1595503..1596372	+	870	WP_006462346.1	ABC transporter permease	-
LAZ82_RS07090 (LAZ82_07090)	1596409..1597179	+	771	WP_234295919.1	SDR family oxidoreductase	-
LAZ82_RS07095 (LAZ82_07095)	1597238..1597774	+	537	WP_039874638.1	cupin domain-containing protein	-
LAZ82_RS07100 (LAZ82_07100)	1597792..1598679	+	888	WP_234295920.1	alpha/beta hydrolase	Toxin
LAZ82_RS07105 (LAZ82_07105)	1598694..1599527	+	834	WP_006462350.1	IclR family transcriptional regulator	Antitoxin
LAZ82_RS07110 (LAZ82_07110)	1599529..1600353	+	825	WP_039874641.1	SDR family oxidoreductase	-
LAZ82_RS07115 (LAZ82_07115)	1600346..1602085	+	1740	WP_234295921.1	thiamine pyrophosphate-binding protein	-
LAZ82_RS07120 (LAZ82_07120)	1602096..1602896	+	801	WP_234295922.1	aspartate dehydrogenase	-
LAZ82_RS07125 (LAZ82_07125)	1602954..1604429	+	1476	WP_234296820.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-279)

Antitoxin

• Transcription regulator IclR, N-terminal (20-68)

Sequences

Toxin

Protein

• Length: 296 a.a.
• Molecular weight: 31337.63 Da
• Isoelectric Point: 6.9441

>T216800 WP_234295920.1 NZ_CP083589:1597792-1598679 [Herbaspirillum frisingense]
MHPHTSIASATAVSCDDALREPLALLEGRFAEGSVEVPGGRVSYRSCGSGPVVVLLHGISSGAASWLQCALKLESQARVI
AWNAPGYGRSTPLTMSQPKAVDYARRLQDMLQALEVEQCVLVGHSLGALMAAAYLAHGDGRARRTLLADPALGYGAAGKQ
ERAHQVREERLGALRTLGIAGMAQKPTRLLSAHASAQDHAWVRWNMQWLDASGYTQAVQLLCGDDIAPYLQAAPRRGLSV
ACGAEDIVTTPEASEGLARQFELPFKLVDAAGHACYIEQPSQFAALVSAQLTSTN

Nucleotide

• Length: 888 bp

>T216800 NZ_CP113486:c212726-211404 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
TGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGGAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGGTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGCCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30744.22 Da
• Isoelectric Point: 6.2489

>AT216800 WP_006462350.1 NZ_CP083589:1598694-1599527 [Herbaspirillum frisingense]
MNKPVMPEEESQDKYSVPGLERGLRLLCEFSRQDKTLSAPELARRLDIPRSTVFRLLATLERMGFVERADGGRDYRLGMS
VLRLGFEYLASLELTEIGRPLLDRLRDEIGYSCNLVVRDGRSIVYVAKSVTQTAFTSHVNVGTRLPAHATVLGRVLLEDL
SLPQLRALYPEEHLEVFSSNTPKTVVELFDMVQADKERGFVLQEGFFETSISTVAAPVRDHSGNVVAAMGATIPSPHIDA
SQLDIIVARVRDTAAELSRLLDHAPQGGAKVVNMWRG

Nucleotide

• Length: 834 bp

>AT216800 NZ_CP113486:c212992-212726 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1V2VDX6