Detailed information of TA system TA216799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 56925..57502
• Replicon: chromosome
• Accession: NZ_CP083589
• Organism: Herbaspirillum frisingense strain AU14559

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LAZ82_RS00235
• Protein ID: WP_234295169.1
• Coordinates: 56925..57290 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7G6AQT8
• Locus tag: LAZ82_RS00240
• Protein ID: WP_006465343.1
• Coordinates: 57284..57502 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAZ82_RS00210 (LAZ82_00210)	52617..52868	+	252	WP_102664360.1	FeoA family protein	-
LAZ82_RS00215 (LAZ82_00215)	52865..54691	+	1827	WP_234295166.1	ferrous iron transporter B	-
LAZ82_RS00220 (LAZ82_00220)	54688..54972	+	285	WP_234295167.1	hypothetical protein	-
LAZ82_RS00225 (LAZ82_00225)	55000..56205	-	1206	WP_006465340.1	threonine ammonia-lyase	-
LAZ82_RS00230 (LAZ82_00230)	56553..56903	+	351	WP_234295168.1	hypothetical protein	-
LAZ82_RS00235 (LAZ82_00235)	56925..57290	-	366	WP_234295169.1	type II toxin-antitoxin system VapC family toxin	Toxin
LAZ82_RS00240 (LAZ82_00240)	57284..57502	-	219	WP_006465343.1	ribbon-helix-helix protein, CopG family	Antitoxin
LAZ82_RS00245 (LAZ82_00245)	57644..58216	+	573	WP_006465344.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
LAZ82_RS00250 (LAZ82_00250)	58249..59115	-	867	WP_234295170.1	ABC transporter permease	-
LAZ82_RS00255 (LAZ82_00255)	59124..59987	-	864	WP_234295171.1	ABC transporter ATP-binding protein	-
LAZ82_RS00260 (LAZ82_00260)	59968..60993	-	1026	WP_234295172.1	ABC transporter substrate-binding protein	-
LAZ82_RS00265 (LAZ82_00265)	60995..62035	-	1041	WP_234295173.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13911.15 Da
• Isoelectric Point: 9.3922

>T216799 WP_234295169.1 NZ_CP083589:c57290-56925 [Herbaspirillum frisingense]
MVKALFDTNILVDYLNGIVAAKKEMARYEYRAISIITWMEVMVGASEDNEEALRSWLSSFHLINLDSTVAQRAVMIRQQR
RIRLPYAIVWASAQEHSLLLVSRNTKDFPANEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T216799 NZ_CP113486:107640-107816 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8314.56 Da
• Isoelectric Point: 7.2019

>AT216799 WP_006465343.1 NZ_CP083589:c57502-57284 [Herbaspirillum frisingense]
MSRILVDIPDSQLAELTQIVDSQQRPRAVVIREAIAAYIAQHKPEHDAEVFGLWKKKKVDGLAYQKELRSEW

Nucleotide

• Length: 219 bp

>AT216799 NZ_CP113486:107862-108278 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7G6AQT8