Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 80769..81370 Replicon plasmid pM121-2
Accession NZ_CP083587
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LA372_RS25525 Protein ID WP_001216034.1
Coordinates 80769..81149 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LA372_RS25530 Protein ID WP_001190712.1
Coordinates 81149..81370 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS25745 75894..75995 - 102 Protein_72 transcriptional regulator -
LA372_RS25500 (LA372_25525) 76209..77651 - 1443 WP_223656860.1 terminase -
LA372_RS25505 (LA372_25530) 77693..78886 - 1194 WP_000219605.1 terminase -
LA372_RS25510 (LA372_25535) 78973..79425 - 453 WP_001312282.1 late promoter-activating protein (Gp10) -
LA372_RS25515 (LA372_25540) 79514..80557 - 1044 WP_224205067.1 DUF968 domain-containing protein -
LA372_RS25520 (LA372_25545) 80585..80764 - 180 WP_000113018.1 hypothetical protein -
LA372_RS25525 (LA372_25550) 80769..81149 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LA372_RS25530 (LA372_25555) 81149..81370 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LA372_RS25535 (LA372_25560) 81443..81832 - 390 WP_000506726.1 S24 family peptidase -
LA372_RS25540 (LA372_25565) 81956..82207 - 252 WP_001283837.1 DNA polymerase III subunit theta -
LA372_RS25545 (LA372_25570) 82240..82590 + 351 WP_000551789.1 hypothetical protein -
LA372_RS25550 (LA372_25575) 82575..83720 - 1146 WP_224205068.1 hypothetical protein -
LA372_RS25555 (LA372_25580) 83702..84076 - 375 WP_000988658.1 hypothetical protein -
LA372_RS25560 (LA372_25585) 84083..84376 - 294 WP_050153512.1 hypothetical protein -
LA372_RS25565 (LA372_25590) 84555..84788 - 234 WP_000516537.1 hypothetical protein -
LA372_RS25570 (LA372_25595) 84871..85284 - 414 Protein_87 DUF551 domain-containing protein -
LA372_RS25575 (LA372_25600) 85456..85635 - 180 Protein_88 hypothetical protein -
LA372_RS25580 (LA372_25605) 85651..85779 - 129 Protein_89 hypothetical protein -
LA372_RS25585 (LA372_25615) 86113..86291 - 179 Protein_90 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94565 94565


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T216798 WP_001216034.1 NZ_CP083587:c81149-80769 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T216798 NZ_CP113485:c3399144-3398683 [Mycobacterium avium subsp. paratuberculosis]
GTGGCCAAGCTTTCCGGATCCATCGATGTTCCGCTGCCCCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGATCGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAAGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGGATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGTTCCGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCCATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT216798 WP_001190712.1 NZ_CP083587:c81370-81149 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT216798 NZ_CP113485:c3399336-3399148 [Mycobacterium avium subsp. paratuberculosis]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATGGCCATCGACAAGGCCGGCGA
GTTCGTCGACGAGAAGACGCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCG
CCACCGAGACCGGCGACCAGCAGGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References