Detailed information of TA system TA216797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 254497..255098
• Replicon: plasmid pNDM-M121
• Accession: NZ_CP083586
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LA372_RS24465
• Protein ID: WP_033551393.1
• Coordinates: 254497..254877 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: LA372_RS24470
• Protein ID: WP_001190712.1
• Coordinates: 254877..255098 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS24420 (LA372_24440)	249889..250098	+	210	WP_000874156.1	hypothetical protein	-
LA372_RS24425 (LA372_24445)	250209..251060	+	852	WP_000611664.1	phage repressor protein C1	-
LA372_RS24430 (LA372_24450)	251093..251425	-	333	WP_224205061.1	phage antirepressor KilAC domain-containing protein	-
LA372_RS24435 (LA372_24455)	251546..251896	-	351	Protein_267	ORF6N domain-containing protein	-
LA372_RS24440 (LA372_24460)	252205..252426	-	222	WP_072019028.1	host cell division inhibitor Icd-like protein	-
LA372_RS24445 (LA372_24465)	252445..252564	-	120	WP_001376634.1	ash family protein	-
LA372_RS24450 (LA372_24470)	252786..253118	-	333	WP_033551395.1	hypothetical protein	-
LA372_RS24455 (LA372_24475)	253242..254285	-	1044	WP_033551394.1	DUF968 domain-containing protein	-
LA372_RS24460 (LA372_24480)	254313..254492	-	180	WP_000113018.1	hypothetical protein	-
LA372_RS24465 (LA372_24485)	254497..254877	-	381	WP_033551393.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LA372_RS24470 (LA372_24490)	254877..255098	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LA372_RS24475 (LA372_24495)	255281..255457	+	177	Protein_275	type I restriction-modification system subunit M N-terminal domain-containing protein	-
LA372_RS24480 (LA372_24500)	255515..255921	+	407	Protein_276	IS1-like element IS1B family transposase	-
LA372_RS24485 (LA372_24505)	255955..257706	-	1752	Protein_277	Tn3-like element TnAs1 family transposase	-
LA372_RS24490 (LA372_24510)	257710..258102	+	393	WP_001351729.1	isochorismatase family cysteine hydrolase	-
LA372_RS24495 (LA372_24515)	258240..259124	+	885	WP_000058717.1	EamA family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaNDM-5 / lnu(F) / blaTEM-1B / tet(A) / qnrS1 / aadA5 / sul2 / ARR-3 / blaOXA-10 / dfrA14 / floR	-	1..372134	372134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13559.26 Da
• Isoelectric Point: 5.6277

>T216797 WP_033551393.1 NZ_CP083586:c254877-254497 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGGISVSSVADTLRRLYGSSE

Nucleotide

• Length: 381 bp

>T216797 NZ_CP113485:2191452-2192030 [Mycobacterium avium subsp. paratuberculosis]
ATGGAGCGACTCGACGTGGTGGTCGGGCCCAACGGCGCCGGCAAGTCGACGTTCATCGCGTTGACCCTGGCGCCGCTGCT
GCCGGGCAGCGTGGTGGTCAACGCCGACGAGATCGCGCGGCAGCGCTGGCCGCAGGATCCGGCGTCGCACGCCTACGACG
CCGCGAAGGTCGCCGCCGACACCCGGGCGAAGCTGATCGACCTGGGCCGGTCGTTCATCGCCGAGACCGTGTTCTCCCAC
CCGTCCAAGCTGGACCTGCTGCACGCCGCCCGCCGCGCCGGCTTCACCGTCGTGCTGCACGTGCTGCTCATCCCGGAAGA
CCTTGCCGTCGAACGGGTCCGGCACCGCGTGCGGGCCGGCGGCCATGACGTGCCGGAAACCAAGATCCGCGAGCGGCATC
GCCGGTTGTGGACTCCGGTCGCCGAGGCGATGACCCTGGCCGACCTGGCCACCGGCTACGACAACAGCCGGCTGCGCGGG
CCGCGTGTGGTGGCCCGGCTCAGCGGCGGGCTGACGGTCGGTGCCGTCGACTGGCCGGCGTGGGCACCCGAGACCTTGCG
GTCGCGTTGGCCGGTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT216797 WP_001190712.1 NZ_CP083586:c255098-254877 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT216797 NZ_CP113485:2191032-2191421 [Mycobacterium avium subsp. paratuberculosis]
ATGGCCGAGGCCGTCGATCGGGTCACCCGGGTCGCGTCCGATCTGATGGACAGCGCGGCGGCCGAGGGCGCCCGTCAGAG
CCGCTCGGCCAAGCAGCAGCTCGACCACTGGGCGCGGGTCGGGCGTGCGGTGTCCAGCCAGCACACCGCGTCCCGGCGGC
GGGTCGAAGCCGCGCTGGCCGGCCGGCTGTCCACCGCCGAACTCACCGCCGAAGAGGGCGTGGTGTTCAACGCCGAGATC
TCGGCGGCCATCGACGAAAGCCTGGCGCGCACCAACTACGGGGCGACGCTGGCCGGGCAGGGTGTCACCACGGTGGCCCT
CAACGACGATGGCGAGATCGTCGAGCACCGGCCCGACGGCACCGCGGTGGTGCTGGCCGGCCGGCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6