Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 84083..85706 Replicon plasmid pNDM-M121
Accession NZ_CP083586
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag LA372_RS23500 Protein ID WP_000088046.1
Coordinates 84381..85706 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag LA372_RS23495 Protein ID WP_024136327.1
Coordinates 84083..84376 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS23470 (LA372_23485) 79955..81196 + 1242 WP_001053338.1 VWA domain-containing protein -
LA372_RS25735 81287..81418 + 132 WP_000374059.1 hypothetical protein -
LA372_RS23475 (LA372_23490) 81759..82040 - 282 WP_000077926.1 hypothetical protein -
LA372_RS23480 (LA372_23495) 82090..82281 - 192 WP_000398480.1 hypothetical protein -
LA372_RS23485 (LA372_23500) 82373..82714 - 342 WP_001151575.1 hypothetical protein -
LA372_RS23490 (LA372_23505) 83087..83479 + 393 WP_000341066.1 hypothetical protein -
LA372_RS23495 (LA372_23510) 84083..84376 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
LA372_RS23500 (LA372_23515) 84381..85706 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
LA372_RS23505 (LA372_23520) 85767..85973 + 207 WP_000134171.1 hypothetical protein -
LA372_RS23510 (LA372_23525) 86075..86485 + 411 WP_000985911.1 hypothetical protein -
LA372_RS23515 (LA372_23530) 86498..86743 + 246 Protein_84 HNH endonuclease -
LA372_RS23520 (LA372_23535) 86796..87500 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LA372_RS23525 (LA372_23540) 87547..88122 - 576 WP_072740834.1 recombinase family protein -
LA372_RS23530 (LA372_23545) 88248..88598 - 351 WP_000454193.1 DUF3330 domain-containing protein -
LA372_RS23535 (LA372_23550) 88801..89814 - 1014 WP_000845048.1 class 1 integron integrase IntI1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaNDM-5 / lnu(F) / blaTEM-1B / tet(A) / qnrS1 / aadA5 / sul2 / ARR-3 / blaOXA-10 / dfrA14 / floR - 1..372134 372134


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T216796 WP_000088046.1 NZ_CP083586:84381-85706 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T216796 NZ_CP113485:1893798-1894205 [Mycobacterium avium subsp. paratuberculosis]
ATGACTGCAAGGCCGGCGGCCGGGGTGCTCGACACCTCGGTGTTCATCGCGTCCGAAAGCGGACGGCAGCTGGACGAGGC
GCGTATCCCCGACGAGGTAGCCACCACCGTCGTCACCCTTGCCGAACTTCACGCCGGCGTATTAGCCGCGACGACGTCCA
ACGTTCGGGCGCAACGCCTCGCAACGCTGGAATCGATAGCGGATATGGAAATGCTGCCAGTCGACGACGACGCGGTCCGC
ATGTGGGCAAGGTTGCGGATCCACCTAGCCGAGGCTGGACGGCGGATGCGGGCTAATGATCTGTGGATCGCGGCGATCGC
GGCATCGCGGGGACTGCCCGTTGTCACCCAGGATGACGACTTCGCCGCACTCGACGGCGCGGCGAACCTGGCGATTATTC
GGGTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT216796 WP_024136327.1 NZ_CP083586:84083-84376 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT216796 NZ_CP113485:1893541-1893801 [Mycobacterium avium subsp. paratuberculosis]
ATGTCTGAAGTGGCGTCTCGCGAGCTGCGCAACGATACGGCGGGGGTACTGCGGCGCGTACGGGAAGGGGAGGACGTCAC
CATCACCATCAACGGCCGAGCCGTCGCGATGCTGACCCCGCTGCGGCCGCAGCGACGGCGGTGGCTGAGCAAGGCGGAGT
TGCTGTCGCGCTTGCGCCGCGCGCAAGCCGATCCCGGGCTACGCCAAGACCTCGCAACCCTTGCAGGCGACACCACTGAG
GACCTCGGTCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References