Detailed information of TA system TA216794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4194797..4195392
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A8S7NE19
• Locus tag: LA372_RS20225
• Protein ID: WP_044189483.1
• Coordinates: 4194797..4195147 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: LA372_RS20230
• Protein ID: WP_001223208.1
• Coordinates: 4195141..4195392 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS20205 (4190243)	4190243..4191265	-	1023	WP_021578254.1	ABC transporter permease	-
LA372_RS20210 (4191279)	4191279..4192781	-	1503	WP_224204952.1	sugar ABC transporter ATP-binding protein	-
LA372_RS20215 (4192921)	4192921..4193877	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LA372_RS20220 (4194187)	4194187..4194717	+	531	WP_000055072.1	inorganic diphosphatase	-
LA372_RS20225 (4194797)	4194797..4195147	-	351	WP_044189483.1	endoribonuclease toxin ChpB	Toxin
LA372_RS20230 (4195141)	4195141..4195392	-	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LA372_RS20235 (4195604)	4195604..4195945	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LA372_RS20240 (4195948)	4195948..4199727	-	3780	WP_047624313.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12541.47 Da
• Isoelectric Point: 5.6219

>T216794 WP_044189483.1 NZ_CP083585:c4195147-4194797 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASSHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T216794 NZ_CP113446:c5335-4940 [Acinetobacter baumannii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAATAAGCCTCAACACGTTTTTGAACGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCAAGTATCACGGCATCTGAGCTGGCTTTCGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAACAGGCGTTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGATCAAGCTATTTGGCATTACGCT
AAATTACGACAGGATCTTCAATCGACAGGTAAACCGATTGGCAGCTTAGATATGCTGATTGCTGCTCATGCCTTGGCGTT
GGATGTTGTATTAGTCACAAACAATACAAAAGAATTTGAACGAATCGATGGGCTAAAGCTAGAGAATTGGGTCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT216794 WP_001223208.1 NZ_CP083585:c4195392-4195141 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT216794 NZ_CP113446:c5562-5332 [Acinetobacter baumannii]
ATGGAAATTGCGAAAGTATTTCAAACTGGTCGCAGTCAAGCGGTTCGCTTGCCTAAAGCCTTTCGCTTTAATGGTACGGA
AGTGGCCATCAAAAGCTTCGGGCGTGGTGTATTACTCATGCCCATCGACAATCCTTGGGATATCATGTTAGAAGCCATCA
ATGAGTTTGAGGTGGGATTCCAGTTGGAGCGAGCTGATCAAGGCGAACAGTTCCGTGAGGAATTCAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26