Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4128515..4129091 Replicon chromosome
Accession NZ_CP083585
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LA372_RS19880 Protein ID WP_001297643.1
Coordinates 4128804..4129091 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LA372_RS19875 Protein ID WP_000063156.1
Coordinates 4128515..4128817 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS19860 (4125111) 4125111..4127261 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LA372_RS19865 (4127311) 4127311..4127514 + 204 WP_000467859.1 YbdD/YjiX family protein -
LA372_RS19870 (4127525) 4127525..4128481 + 957 WP_001297640.1 GTPase -
LA372_RS19875 (4128515) 4128515..4128817 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LA372_RS19880 (4128804) 4128804..4129091 - 288 WP_001297643.1 BrnT family toxin Toxin
LA372_RS19885 (4129279) 4129279..4129976 - 698 Protein_3894 IS1 family transposase -
LA372_RS19890 (4130033) 4130033..4130128 + 96 Protein_3895 dienelactone hydrolase family protein -
LA372_RS19895 (4130273) 4130273..4131505 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
LA372_RS19900 (4131546) 4131546..4132826 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4129279..4129488 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T216793 WP_001297643.1 NZ_CP083585:c4129091-4128804 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T216793 NZ_CP113445:6328-6648 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT216793 WP_000063156.1 NZ_CP083585:c4128817-4128515 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT216793 NZ_CP113445:6641-6913 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References