Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4017869..4018419 Replicon chromosome
Accession NZ_CP083585
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag LA372_RS19330 Protein ID WP_001160971.1
Coordinates 4017869..4018183 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag LA372_RS19335 Protein ID WP_000125566.1
Coordinates 4018186..4018419 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS19305 (4013544) 4013544..4014830 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
LA372_RS19310 (4014830) 4014830..4015819 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
LA372_RS19315 (4015816) 4015816..4016637 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
LA372_RS19320 (4016640) 4016640..4017017 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
LA372_RS19325 (4017024) 4017024..4017872 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
LA372_RS19330 (4017869) 4017869..4018183 - 315 WP_001160971.1 CcdB family protein Toxin
LA372_RS19335 (4018186) 4018186..4018419 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LA372_RS19340 (4018505) 4018505..4018984 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
LA372_RS19345 (4019176) 4019176..4021038 - 1863 WP_113417176.1 glutathione-regulated potassium-efflux system protein KefC -
LA372_RS19350 (4021031) 4021031..4021561 - 531 WP_000600735.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
LA372_RS19355 (4021669) 4021669..4023000 - 1332 WP_001183198.1 MFS transporter -
LA372_RS19360 (4023059) 4023059..4023346 - 288 WP_113417175.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T216792 WP_001160971.1 NZ_CP083585:c4018183-4017869 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T216792 NZ_CP113444:c8622-8320 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTTGGTGAAGGTGTCTCTGAATTACGTTTCT
TCTTTGGACCAGGCTATAGGATTTACTACTGCAAACAAGGGCAAAGGGTTGTTATTCTTTTATCAGGTGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT216792 WP_000125566.1 NZ_CP083585:c4018419-4018186 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT216792 NZ_CP113444:c8319-8038 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAGGCTTACTTACAAGTATG
TATTGAAGAATCTAATGGTGATGCCGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACGGTG
ATTAAAGTTGTAAAAGCACTGAATTTGCGCTTAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References