Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3547094..3547712 Replicon chromosome
Accession NZ_CP083585
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LA372_RS16940 Protein ID WP_001291435.1
Coordinates 3547494..3547712 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LA372_RS16935 Protein ID WP_000344800.1
Coordinates 3547094..3547468 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS16925 (3542183) 3542183..3543376 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LA372_RS16930 (3543399) 3543399..3546548 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LA372_RS16935 (3547094) 3547094..3547468 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LA372_RS16940 (3547494) 3547494..3547712 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LA372_RS16945 (3547884) 3547884..3548435 + 552 WP_000102563.1 maltose O-acetyltransferase -
LA372_RS16950 (3548551) 3548551..3549021 + 471 WP_000136192.1 YlaC family protein -
LA372_RS16955 (3549185) 3549185..3550735 + 1551 WP_047623883.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LA372_RS16960 (3550777) 3550777..3551130 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LA372_RS16970 (3551509) 3551509..3551820 + 312 WP_000409911.1 MGMT family protein -
LA372_RS16975 (3551851) 3551851..3552423 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216789 WP_001291435.1 NZ_CP083585:3547494-3547712 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216789 NZ_CP113442:c3336667-3336278 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCACGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGTAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216789 WP_000344800.1 NZ_CP083585:3547094-3547468 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216789 NZ_CP113442:c3336931-3336674 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References