Detailed information of TA system TA216788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2842414..2844134
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: LA372_RS13800
• Protein ID: WP_000312833.1
• Coordinates: 2842414..2843070 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: LA372_RS13805
• Protein ID: WP_000634203.1
• Coordinates: 2843067..2844134 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS13785 (2838329)	2838329..2838568	+	240	WP_024224214.1	hypothetical protein	-
LA372_RS13790 (2838608)	2838608..2841046	+	2439	WP_032180025.1	type I restriction-modification system subunit M	-
LA372_RS13795 (2841046)	2841046..2842401	+	1356	WP_223349791.1	restriction endonuclease subunit S	-
LA372_RS13800 (2842414)	2842414..2843070	+	657	WP_000312833.1	toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT	Toxin
LA372_RS13805 (2843067)	2843067..2844134	+	1068	WP_000634203.1	toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG	Antitoxin
LA372_RS13810 (2844153)	2844153..2847248	+	3096	WP_000108736.1	HsdR family type I site-specific deoxyribonuclease	-
LA372_RS13815 (2847248)	2847248..2847964	+	717	WP_001122107.1	SprT family zinc-dependent metalloprotease	-
LA372_RS13820 (2848309)	2848309..2848923	+	615	Protein_2711	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T216788 WP_000312833.1 NZ_CP083585:2842414-2843070 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T216788 NZ_CP113442:c760654-760475 [Acinetobacter baumannii]
ATGAGCTTCAACGAGTTTAAACGATGGCTAATTGCACAGGGAGTTATCTTTGTGCGTAAAGGTAAGGGATCACATATGAT
TATTGAATTTAACGGTAAGAAGACTGTTTTCCCTAACCACGGGAAAAAAGAAATTCCTGAAGGCACTCGTTTAAAGATCA
AAAAGGATTTGGGGCTTTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT216788 WP_000634203.1 NZ_CP083585:2843067-2844134 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT216788 NZ_CP113442:c760389-759976 [Acinetobacter baumannii]
ATGAAATGTTATGTCAGTATTCATAGGGAGGGAGAGGCTTTTATAGTCAGCTCAAGTGAACTCCCTGAACTAAATAGTGT
AGGCTATACTCTAGAAGAAGCTTTATCAGAAGCTTTGGATGGTATAGAAACAGTATTCGAAATTTATATGGATGAGCGTA
AAGCCATCCCATTACCATCGAAAGGTAAGAAAGGTGAATATGTAGTTCATCTTCCAGTTCGAGTCGCTGCTAAGGTACGT
TTATATAATGAGATGATCTCACAAAATGTTACTAAAGCTGAATTAGCTCGTCGCTTGGGGTGGTTACAGAAGCAAGCCGA
TCGTTTGCTTTCTTTAAAGCATTCAACAAAACTAGAATCGATTGAAAGTGCCTTCCAAGCATTAGGCAAGGATCTCGATA
TAGTTATCGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9